FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242898
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA116400.35893820897234513No Hit
GTATATTACTGTGCGAAAGCCCTCTTAGTGGCTACGATTACCTCGCGGAA78350.24160488550672887No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC72110.2223628371906856No Hit
GATTATTACTGCAGCTCATATGGAGGCAGGAAAAATTTGGTGTTCGGCGG70010.21588714785355567No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG69140.2132043622710304No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT64340.19840278664330482No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA56770.17505946841374598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.17105070834790365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT53490.16494505840146684No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51070.15748259735582187No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTTTTCCTG50300.15510817793220755No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG48280.14887918152220636No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG46940.14474707499279965No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC46010.14187926971492784No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT44750.13799385611264986No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA44750.13799385611264986No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA44410.13694541117235262No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA43980.13561943668903556No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43490.1341084425103719No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT42940.13241242863636168No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42160.13000717259685626No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT42000.12951378674259875No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT40230.12405570572987495No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA39460.12168128630626063No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA39180.12081786106130998No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39140.12069451459774559No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38990.12023196535937918No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA38980.12020112874348808No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAA38930.12004694566403261No Hit
GTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA38680.11927603026675522No Hit
AGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA38590.11899850072373537No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38030.11727165023383405No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37910.11690161084314092No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC37790.11653157145244779No Hit
CATATGGAGGCAGGAAAAATTTGGTGTTCGGCGGAGGGACCAAGCTGACC37790.11653157145244779No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG37680.11619236867764574No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37320.11508225050556632No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36590.1128311775455164No Hit
GTCAGTAAGTGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC36070.11122767351917945No Hit
CTCCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCA35940.11082679751259522No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT35680.11002504549942674No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCT35670.10999420888353566No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34830.10740393314868368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT34430.10617046851303988No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC34250.10561540942700016No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33920.10459780110259403No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT33390.102963460460366No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT33040.10188417890417767No Hit
TTTATGAGGTCAGTAAGTGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGC33020.1018225056723955No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA32880.10139079304992016No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTC32720.10089740719566263No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32580.10046569457318731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)