FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126413
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAAAGATCTCGGGTGGAAGCAGCAGCTGGAAAGGTA242340.7751375138217503No Hit
GTAATATATAGCCATGTCGTCACCTCTAAGGCTGTTCATTTGCAAATGCA150670.48192609229810646No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT110820.35446372568179574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC98890.316304979540451No Hit
ATATAAGAGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG86430.27645099991587807No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT82800.2648402498326357No Hit
CCTCTAAGGCTGTTCATTTGCAAATGCAGCGTGTTCTTGGAATTGTCTCT71860.2298480718958116No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC71370.22828078056226098No Hit
ATATTACTGTGCGAAAGATCTCGGGTGGAAGCAGCAGCTGGAAAGGTACT71160.22760908427645354No Hit
CTCTTATATCATATGATGGAAGTAATAAACATTATGCGGACTCCGTGAAG64170.20525119362029262No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG61660.19722282372802313No Hit
TTATTACTGTCAGCAGCGTAACAGCTGGCCTCCGACTTTCGGCGGAGGGA57020.18238153436542132No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55800.17847929880025448No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT55160.17643222440541284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53920.17246601776540718No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA52750.16872370988733734No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52130.16674060656733453No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50130.16034349908345444No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAACAGC48290.15445816019828473No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47180.1509077655447313No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT46750.14953238743569708No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44840.14342314978859158No Hit
GCCTTAGAGGTGACGACATGGCTATATATTACTGTGCGAAAGATCTCGGG44750.143135279951817No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT43860.14028856712149035No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT43220.1382414927266487No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG41830.13379550302535206No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG41250.13194034185502682No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA40010.12797413521502118No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39800.12730243892921375No Hit
GCTATATATTACTGTGCGAAAGATCTCGGGTGGAAGCAGCAGCTGGAAAG39390.12599103189501834No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC39260.12557521990856613No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGGTACTTAGCCTGGTACCGAC38040.12167298434339929No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37950.12138511450662469No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA37650.12042554838404267No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37280.11924208349952485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36940.11815457522726523No Hit
GTTTATTACTGTCAGCAGCGTAACAGCTGGCCTCCGACTTTCGGCGGAGG36830.11780273431565183No Hit
CAGTAATATATAGCCATGTCGTCACCTCTAAGGCTGTTCATTTGCAAATG36700.11738692232919963No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTTTGGCCCTGCT36060.11533984793435799No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC35740.11431631073693718No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG35350.11306887477758056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35050.11210930865499856No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGT34580.11060598839628673No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG34110.10910266813757491No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA34100.10907068260015552No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA34090.10903869706273611No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.10862288507628391No Hit
GTAATAAACATTATGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGA33960.10862288507628391No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG33770.1080151598653153No Hit
CCTCATCTATGATGCCACCAGCAGGGCCAAAGGCATCCCAGCCAGGTTCA33690.10775927556596009No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA33050.10571220117111847No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC33030.10564823009627966No Hit
GTACTTAGCCTGGTACCGACAGAAACGTGGCCAGGCTCCCAGGCTCCTCA32840.10504050488531105No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA32560.10414490983756786No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC32160.10286548834079184No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT32040.10248166189175902No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA31900.1020338643678874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31370.10033863088465919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)