FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288733
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGGCCCGAAATAGTCAGGGAGG88500.26910059284228915No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC68170.20728347360518476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65120.19800938537728663No Hit
GACTATTACTGTTCAACATGGGACTACAGCCTCAGTGCTCAGGTGTTCGG64650.19658026358479086No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT57800.1757515736303312No Hit
GTCCTGTACTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGT57640.17526506408394965No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54200.16480510883674654No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTGAGCACTGAGG50280.15288562495039884No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG50130.15242952225066614No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT48750.14823337741312537No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48470.14738198570695768No Hit
CCTGAGCACTGAGGCTGTAGTCCCATGTTGAACAGTAATAGTCAGCCTCG46690.14196956700346303No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45860.1394457987316088No Hit
GTACTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAA45350.13789504955251763No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA40670.12366464532085761No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA38770.11788734445757683No Hit
ACATGGGACTACAGCCTCAGTGCTCAGGTGTTCGGCGGAGGGACCAAACT37930.11533316933907374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.11484665979269221No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37670.11454259132620374No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC37300.11341753800019642No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36760.11177556828115873No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36390.11065051495515142No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTG36260.11025522594871644No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG35850.10900854523611372No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT35790.10882610415622064No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTACTGGAAACAGCA35600.10824837406989257No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35360.10751860975032027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.10517728255835909No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA33930.10317043067953524No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33600.10216700474012333No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA33010.10037300078784141No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)