FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288733
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74550.22668304176714865No Hit
GTCCTGTACTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGT69750.21208775537570243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.210202530883474No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGGCCCGAAATAGTCAGGGAGG67300.20463807794673508No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC66790.20308732876764393No Hit
GACTATTACTGTTCAACATGGGACTACAGCCTCAGTGCTCAGGTGTTCGG61440.1868196658105112No Hit
GTACTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAA57520.1749001819241635No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT53280.1620076789450527No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG53020.16121710093218272No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT50380.1531896934168873No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA46020.13993230827799033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA45850.13941539188495994No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTACTGGAAACAGCA44430.1350976196608238No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43680.1328171061621603No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTGAGCACTGAGG42250.1284689270913753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39890.12129291128224759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39430.11989419633640067No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA38790.11794815815087453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36910.11223167098089143No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36050.10961668216909065No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35820.10891732469616719No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35290.10730576182377834No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34990.10639355642431295No Hit
CATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGCTC34270.10420426346559601No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTG34210.10402182238570294No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33410.10158927465379526No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA33320.10131561303395563No Hit
ACATGGGACTACAGCCTCAGTGCTCAGGTGTTCGGCGGAGGGACCAAACT33200.10095073087416948No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG32980.10028178024789486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)