FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418157
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA143160.41882218985260183No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA107550.3146432419575812No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT101550.2970899230199198No Hit
GGATTATACTGCCCCCCTTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC96110.28117491384977344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC92700.27119877758686917No Hit
GTATATTACTGTGCGAGACGTGGCCATGGGGTACGAGGGCTTGACTACTG87680.2565125007423591No Hit
GTATAATCCTGTAGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCT80000.2340442525021525No Hit
GTATATGGTACTACTACTACTACTAATGTATGAAATCCACTCCAGCCCCT62520.18290558333043216No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT55990.16380172121994396No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG53640.15692667130269322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50030.14636542440853362No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG48860.14294252721568962No Hit
GCATGGAAATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43920.1284902946236817No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43590.12752486208211034No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43580.12749560655054756No Hit
ACTTATTACTGTCTACAGGATTATACTGCCCCCCTTTTTGGCCAGGGGAC41710.12202482214830974No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40300.11789979219795932No Hit
GGGTTATCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTACGCTCCTGAT39880.11667105987232301No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA38650.11307262949010242No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37970.11108325334383412No Hit
GTCCCAGCCAGGACACAGCATGGAAATGAGGGTCCCCGCTCAGCTCCTGG37460.10959122123413288No Hit
ATTCTGCATCCAGTTTGCAGTCGGGGGTCCCATCTCGGTTCAGTGGCAGT37260.10900611060287752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37160.10871355528724984No Hit
ATCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTACGCTCCTGATCTATT36280.10613906850972614No Hit
GTCTACAGGATTATACTGCCCCCCTTTTTGGCCAGGGGACCAAGCTGGAG36180.10584651319409846No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT35630.10423745895814615No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA35450.10371085939001631No Hit
GCTGTATATTACTGTGCGAGACGTGGCCATGGGGTACGAGGGCTTGACTA34830.10189701643312464No Hit
CTCCTGATCTATTCTGCATCCAGTTTGCAGTCGGGGGTCCCATCTCGGTT34790.10177999430687355No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA34520.1009900949546788No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34370.10055126198123725No Hit
CTACAGGATTATACTGCCCCCCTTTTTGGCCAGGGGACCAAGCTGGAGAT34280.10028796219717234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)