FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004729
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT64610.1613342625680789No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56250.14045894241532947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54290.1355647285996131No Hit
GTATTATTGTGTCACACAGGAGGAGCGATTCAGTGACTTCCAATGGGTGT51460.1284980831412063No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC49850.12447783607829642No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTCCAGATACA49390.1233291940603222No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA47010.11738622014123802No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA45670.11404017600192173No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC44500.11111862999968287No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA43930.10969531271654087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43650.10899613931429568No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTCCAGATA43400.10837187734800532No Hit
ACACTACCCCGTACACCTTTGGCCAGGGGACCAAGCTGGACATCAAACGA42260.1055252427817213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)