FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178869
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144080.453242961569036No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT112930.35525213527201027No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT91890.2890650731439389No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76740.2414066134842298No Hit
ATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA76240.23983372702681363No Hit
GTCTCTGGTGGCTCCGTCAACAGTAGTGAAACTAATATTTATTACTGGGG66700.2098230534193136No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTG61470.19337066107474074No Hit
ATTTATTACTGGGGCTGGATCCGCCAGTCCCCCGGGAAGGGGCTGGAGTG57150.17978092208266525No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA56860.1788686479373639No Hit
ATTATGATGGGTCCACCTACTACAACCCGTCCCTCAAGACTCGAGTCACC56700.17836532427099072No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55150.17348937625300068No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53950.16971444875520192No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC52400.16483850073721187No Hit
ATTTATTATTGTGCGAGAGCGTATTATTTTAATGATTTCGAGTGGTATTT48750.15335642959807405No Hit
ACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACACGTCC48370.15216103589043775No Hit
CAATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGA47440.14923546707964372No Hit
GAGTTACAATCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA47240.14860631249667727No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC47190.14844902385093567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46400.14596386324821814No Hit
AGTCAGAACATTACCAACTTTTTAATTTGGTATCAAAAAAAACCAGGGAA46290.14561782822758662No Hit
TTATTATTGTGCGAGAGCGTATTATTTTAATGATTTCGAGTGGTATTTTG45620.14351016037464898No Hit
ACCTACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACAC45290.1424720553127543No Hit
CACCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC45240.1423147666670127No Hit
ATCTATTATGATGGGTCCACCTACTACAACCCGTCCCTCAAGACTCGAGT45000.14155978116745294No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGGGTCACCATCACTT44270.13926336693962538No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA43200.13589738992075484No Hit
ACTTACTACTGTCAACAGAGTTACAATCTCCCGTGGACGTTCGGCCAAGG43140.1357086435458649No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42260.13294036338081247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42030.13221683561040104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.13205954696465944No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG41410.13026645640320506No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA39650.12472989607310021No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT38960.12255931276186595No Hit
CTATTATGATGGGTCCACCTACTACAACCCGTCCCTCAAGACTCGAGTCA38670.12164703861656458No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC37790.11887875845151216No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT37670.11850126570173228No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA36790.11573298553667988No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36710.11548132370349329No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA36350.11434884545415366No Hit
CCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG34780.10940998197786697No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC34760.10934706651957032No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC34460.10840333464512064No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA34190.10755397595811592No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC34110.10730231412492933No Hit
CTGTAGATGGTGAGAGTGAAATCTGACCCAGACACACCGCCACCGAACCT33990.10692482137514947No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT33950.10679899045855618No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT33350.1049115267096568No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG32510.10226907746119768No Hit
TTACTACTGTCAACAGAGTTACAATCTCCCGTGGACGTTCGGCCAAGGGA32470.10214324654460438No Hit
GTAGTAGGTGGACCCATCATAATAGATAGTCGCAATCCACTCCAGCCCCT32340.10173429606567619No Hit
TCATAATAGATAGTCGCAATCCACTCCAGCCCCTTCCCGGGGGACTGGCG32210.10132534558674798No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTGGCGGT32040.1007905641912265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)