FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907639
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGACAGCACCGTATGACTACGGTGACTACGATCCTCT169610.4340472597391929No Hit
GTAATATACGGCCGTGTCCTCAAATCTCAGGCTGCTCAACTCCATGTAGG159960.40935204096386585No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAAGGATCATCCCTATCCTTCAT117130.29974621504187055No Hit
TCCCTATCCTTCATATAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTC103090.2638165910412912No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT99560.2547830032405757No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT84110.2152450622997672No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT80950.20715833780960832No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA80400.20575083829391608No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC77000.1970499321969097No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT76300.1952585691769378No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG74980.19188057033927647No Hit
CAGCAAGACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC73780.18880966230503893No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA73700.18860493510275644No Hit
GCCGTATATTACTGTGCGACAGCACCGTATGACTACGGTGACTACGATCC71390.18269343713684913No Hit
ATATACGGCCGTGTCCTCAAATCTCAGGCTGCTCAACTCCATGTAGGCTA69460.17775439338178373No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGC68900.1763213029658062No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT66810.1709728048061758No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG65780.16833694207678856No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG65460.16751803326765854No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA65030.1664176245553901No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC61820.15820294556380463No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG61260.15676985514782713No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT60760.15549031013356146No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT60320.15436431052100769No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC59940.1533918563101658No Hit
ATATTACTGTGCGACAGCACCGTATGACTACGGTGACTACGATCCTCTTG57350.14676381313626974No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT52660.134761680902458No Hit
TCCTATGACAGCAGCCTGAGTGGTCTTGCTGTGTTCGGAGGAGGCACCCA52020.13312386328419795No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG51810.13258645437820638No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG51610.1320746363725001No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA51150.13089745495937574No Hit
CCTTCATATAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCGCGATTA49980.1279033196259941No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG49340.12626550200773407No Hit
GTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTC48880.1250883205946097No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA48340.12370641197920278No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCA48250.12347609387663497No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG48130.12316900307321121No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT47010.12030282224125617No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACAC46660.1194071407312702No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG46170.11815318661728988No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA45600.11669450530102704No Hit
AGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGG45470.11636182359731798No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA44860.11480077867991388No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTCTTGCTGTGTT44660.11428896067420763No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA44210.11313737016136853No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC43980.11254877945480635No Hit
CTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATT43630.11165309794482038No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT43430.11114127993911414No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC41980.10743059939774374No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATTTGAGGACACGGCCGTATATTA41610.10648373608718718No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC41540.10630459978518997No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC41210.10546010007577465No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT40630.10397582785922652No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39640.10144232873098052No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG39440.10093051072527426No Hit
TTCCAGGGCAGAGTCGCGATTACCGCGGACAAATCCACGGGCATAGCCTA39410.10085373802441833No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG39320.10062341992185052No Hit
CCGTATGACTACGGTGACTACGATCCTCTTGACTACTGGGGCCAGGGAAC39300.10057223812127988No Hit
ATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTC39240.100418692719568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)