FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905116
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC90260.23113269874697703No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG84420.2161779573257235No Hit
GTAATACACGGCCGTGTCCTCTGATCTCAGGCTGCTCAGCTCCATGTACA78370.20068545979171937No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT74760.19144117613919792No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT72590.18588436297410882No Hit
GTGTACATGGAGCTGAGCAGCCTGAGATCAGAGGACACGGCCGTGTATTA72090.1846039912770837No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA71020.1818639958454499No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAATACCACTTGAAAGCCA69690.17845820713136307No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG68030.1742073730972396No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA65060.16660196521691034No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACACCAAGGGGGACGGGA61480.15743450386621036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.15541151658491065No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT57410.1470122782524258No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA57050.1460904106305677No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG56910.14573190655540066No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC55210.1413786427855152No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA54600.13981658931514454No Hit
GTGCATATAGTAGCTGATGAAAGTGTATCCAGATGCCTTGCAGGAAACCT53830.13784481690172584No Hit
GTTATTATGAGCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC53820.13781920946778534No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53520.13705098644957026No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG53070.13589865192224765No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG52260.1338244497730669No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAATACCAC51960.13305622675485185No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA51080.13080277256808762No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC49810.12755062845764378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49700.12726894668429825No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCT49650.12714090951459572No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA49140.12583493038363008No Hit
GTCTGAGGATGAGGCTGACTATTTTTGTCAGACCTGGGGCACTGGCTTTC48980.12542521144058205No Hit
CCATGTACACTGTGCTCGTGGACGTGTCCCTGGTCATGATGACTCTGCCC48560.12434969921508093No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG48210.12345343902716334No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA47390.12135362944404211No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT45270.11592485344865555No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG44560.11410672563887986No Hit
ACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAATACCACTTGAAAGCCAG44390.11367139926189132No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC43560.11154598224482959No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT43490.11136673020724608No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT43290.11085458152843602No Hit
CTCCCAGGTGCAGCTGGAGCAGTCTGGGGCTGAGGTGAGGAAGCCTGGGG43120.11041925515144749No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA42830.1096766395671729No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT42630.10916449088836286No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC42530.10890841654895783No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41770.10696225156947962No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTTCTGCTGATGCCATGCGATGGC41470.10619402855126453No Hit
ACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGAA40760.10437590074148886No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTTCTGCTGATG40760.10437590074148886No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT40700.10422225613784585No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC40000.10242973576201064No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT39560.10130300866862854No Hit
GCTTAACAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT39220.10043235591465145No Hit
GTGTATTACTGTGCGAGAGCTGGGCGGGGGGGGGATATTTTGAGTGGTTA39200.10038114104677044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)