FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200106
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGCGAGACATCCCAAGTATAGCAGCTCGTCGGGGGCATC136440.3248489442885489No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT99510.23692259195363163No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG88150.20987565551917023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86490.2059233743148387No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGA84910.2021615644938485No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT75210.17906690926371857No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTA70730.16840051179660703No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC67410.160495949387944No Hit
GTATTACTGTGCGAGACATCCCAAGTATAGCAGCTCGTCGGGGGCATCGT66860.15918645862747274No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC63540.15128189621880972No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT63020.15004383222709142No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA62440.14866291469786716No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA62430.1486391057749495No Hit
CATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCC58530.1393536258370622No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT57140.1360441855515075No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC55530.1322109489617643No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG54590.1299729102075043No Hit
CCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTG50750.12083028380712296No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA50640.1205683856550287No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50420.12004458935084018No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG50110.11930651274039274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48840.11628277952984996No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT48810.11621135276109698No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT48310.11502090661521398No Hit
GCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACATCCCAAG47920.11409235862142528No Hit
TCTCCAAGGAGTCTGTGCCGAGGTGCAGCTGGTGCAGTCTGGAGCAGAGG45170.10754490481906884No Hit
GTATAGCAGCTCGTCGGGGGCATCGTACGACTACTGGGGCCAGGGAACCC44140.1050925857585499No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43220.1029021648501252No Hit
ATCATGGGGTCAACCGCCATCCTCGCCCTCCTCCTGGCTGTTCTCCAAGG43190.10283073808137223No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG43100.1026164577751133No Hit
GATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCC43010.10240217746885436No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42180.10042603686668859No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)