FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211061
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCGACGTTCGGCCAAGGGACCAACGTGGAAATCAAAC119170.37112343863912894No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT98840.30781103192994463No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA91410.28467226253253985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86320.2688208040893648No Hit
TTGTATTACTGTGCAAAAGGCGGGGGAGCTACTATTACCTTTGACTACTG77790.24225637569638198No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC66500.2070966574599486No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGACCA59470.18520358224275402No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT58150.18109279144806031No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC56640.17639029591776675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55320.17227950512307302No Hit
GTGGTAGCACCTACTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCC54830.1707535297523155No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT52140.1623762363904018No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT50900.15851458443175015No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50540.15739345966956092No Hit
ACTATTACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC49870.1553069219177088No Hit
CATCTATGGTGCATCTAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTG49250.153376095938383No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC46590.14509222963998503No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT44850.13967345995607058No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA44390.13824091164882885No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43280.13478411029874549No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT42320.13179444426624096No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41020.12774593818055777No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT40990.127652511117042No Hit
CTCTTATTAGTTGGGACGGTGGTAGCACCTACTATGCAGACTCTGTGAAG39510.12304344265026419No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC38230.1190572212735915No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38020.11840323182898115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37810.11774924238437078No Hit
TAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGTCTGGAGT37460.11665925997668683No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37440.11659697526767632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36900.11491528812439253No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC36230.11282875037254042No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36120.1124861844729826No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG35330.11002593846706743No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG35140.10943423373146756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCC34810.10840653603279415No Hit
GTGCTACCACCGTCCCAACTAATAAGAGAGACCCACTCCAGACTCTTCCC33810.1052923005822686No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT33770.10516773116424757No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33670.10485630761919502No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG33630.104731738201174No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGAC33490.10429574523810044No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT33370.10392203698403736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33350.10385975227502685No Hit
GGTAATAGTAGCTCCCCCGCCTTTTGCACAGTAATACAAGGCGGTGTCCT33180.1033303322484375No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCCCTTGGCCGAACGTC33170.10329918989393225No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGC32840.10227149219525884No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA32470.10111922507856437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)