FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211061
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCGACGTTCGGCCAAGGGACCAACGTGGAAATCAAAC137600.42851879799231474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT96910.30180055751043033No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA85860.2673882557821231No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC72710.2264360596077122No Hit
TTGTATTACTGTGCAAAAGGCGGGGGAGCTACTATTACCTTTGACTACTG72510.22581321251760714No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGACCA71230.2218269911409344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66400.20678523391489603No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT59820.18629356465043798No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT57780.17994052433136587No Hit
GTGGTAGCACCTACTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCC51870.1615353928187599No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51550.16053883747459174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50130.15611662313484545No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC49040.15272210649377263No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT48940.15241068294872007No Hit
ACTATTACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC47770.14876702747160517No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47140.14680505913777409No Hit
CATCTATGGTGCATCTAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTG45370.1412928623903439No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44310.1379917728127868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44070.13724435630466067No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43980.13696407511411338No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA43910.1367460786325766No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT42970.13381869730908258No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT42520.13241729135634608No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41850.13033075360449398No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA41610.12958333709636782No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC40700.1267493828363896No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC39570.12323029677729573No Hit
CTCTTATTAGTTGGGACGGTGGTAGCACCTACTATGCAGACTCTGTGAAG39250.12223374143312757No Hit
TAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGTCTGGAGT39190.12204688730609603No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT38540.12002263426325444No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGAC36540.11379416336220333No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT36440.11348273981715079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36340.11317131627209823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA35850.1116453409013407No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG35370.11015050788508844No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT34540.10756569246115225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA33630.104731738201174No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA33560.1045137417196372No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC33280.10364175579349007No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC33260.10357947108447955No Hit
TTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCT33200.10339261695744802No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCC32890.1024272039677851No Hit
GGTAATAGTAGCTCCCCCGCCTTTTGCACAGTAATACAAGGCGGTGTCCT32820.10220920748624833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32760.10202235335921679No Hit
CCTTTAATAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGT32200.10027838150692248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)