FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005107762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089411
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATTACTGCACCTCATATACAACCACCAACACTCGAGTGTTCGGCGG100430.24558548896161328No Hit
GTAATAAGTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG89850.21971379252415565No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC87470.2138938834956917No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT82750.20235187903588073No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT67790.165769593714107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67710.1655739665198729No Hit
GTATATTTCTGTGCGAGAGAGGTCTCCCATTGTCGTGGGGGCTTGTGCTA66540.1627129188041994No Hit
GAAATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGGAGATTCA65250.15955843029717484No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT63170.15447212324708864No Hit
CTCCTGGACAGTCGATCACCATCTCGTGCACCGGAACCGACAGTGACGTT62360.15249139790546853No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC61670.15080411335519955No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA60320.14750290445249942No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC59380.14520428492024892No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA59090.14449513634115035No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54900.1342491620431402No Hit
GACCTAGGACGGTCAGCTGGGTCCCGCCGCCGAACACTCGAGTGTTGGTG54620.1335644668633209No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC54010.132072809507286No Hit
ATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGGAGATTCACTA53870.13173046191737636No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAATTGGTGGAGTCTGGGGGAAGGT53280.1302877113599No Hit
ATATTACGCAGACTCCGTGAAGGGCAGATTCTCCATCTCCAGAGACAATT53100.1298475501728733No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52980.12955410938152218No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA52650.12874714720530658No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG52270.12781791803269468No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA51700.12642407427377683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48450.11847671950801716No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT48010.11740076993972971No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA47850.11700951555126154No Hit
CTATAACTATGTCTCCTGGCACCAACAACTCCCAGGCAAAGCCCCCAAAC46700.11419737463414657No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46610.11397729404063323No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT45700.11175203470622053No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC44980.10999138995811378No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC44140.1079373044186559No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT43870.10727706263811586No Hit
CAATTATTGATAGTGGCGGTAGGAAATATTACGCAGACTCCGTGAAGGGC43770.10703252864532324No Hit
CCTCATATACAACCACCAACACTCGAGTGTTCGGCGGCGGGACCCAGCTG42910.10492953630730686No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT42700.10441601492244237No Hit
GGCTATAACTATGTCTCCTGGCACCAACAACTCCCAGGCAAAGCCCCCAA42520.10397585373541568No Hit
GCTGTATATTTCTGTGCGAGAGAGGTCTCCCATTGTCGTGGGGGCTTGTG41880.10241083618154302No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC41780.1021663021887504No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCGCCGCCGAACACTCG40910.10003885645145473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)