FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041510
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCGCGGCGGTCACGGAGCTCAGCCTCAGGGAGA187930.4649994680206161No Hit
GTCTATGGTGCGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC161640.39994952381659327No Hit
GTCTATTACTGTGCGAGCTTTTATTACTATGGTACTAGTGGTTATCACTA148750.36805550400716563No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC95540.23639679228803098No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT91040.22526234006596543No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT83290.2060863390168526No Hit
GTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGA82230.20346355693787718No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA81060.20056859936014013No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT74180.18354525907396No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA74060.18324834034803825No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG71570.17708727678516198No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAG69390.17169325326425025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT68800.17023340286180166No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA67950.1681302285531893No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA67940.16810548532602915No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG66260.16394862316312467No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC63940.1582081944619709No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62270.1540760755262266No Hit
GTACTAGTGGTTATCACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCC61150.1513048340842903No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTGCTG59670.14764283646458873No Hit
ATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC58300.1442530143436488No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT58110.14378289302760602No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA57930.14333751493872338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT57610.14254573166959875No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG57100.14128382708443132No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG56930.14086319222270882No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT56090.13878476114125662No Hit
ACTATGTGCAGTGGTTCCAGCAACGCCCGGGCAGTTCCCCCACCACTGTG54700.13534545256599637No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC54700.13534545256599637No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA54450.13472687188699273No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53870.13329176471170429No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT52840.1307432123142093No Hit
GACTACTACTGTCAGTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGG51860.12831837605251503No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG51740.12802145732659329No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA51470.12735339019326936No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA50750.12557187783773888No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA48810.12077169176867063No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA48810.12077169176867063No Hit
CTACTACTGTCAGTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGGAG48670.12042528658842858No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA48410.11978196268226479No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA46880.11599624892676252No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGA46760.11569933020084076No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT46710.11557561406504004No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC46600.11530343856627845No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG46430.11488280370455597No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC46280.11451165529715378No Hit
CTATTACTGTGCGAGCTTTTATTACTATGGTACTAGTGGTTATCACTACT46260.11446216884283349No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG45750.11320026425766608No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA45630.11290334553174433No Hit
CAGTAATAGACAGCCGTGTCCGCGGCGGTCACGGAGCTCAGCCTCAGGGA45270.11201258935397908No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG44710.11062696863301093No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG44640.11045376604288991No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC44280.10956300986512467No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA44160.10926609113920292No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG43790.10835059173427754No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG43780.10832584850711739No Hit
CCCAATGATTGCTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC43020.10644536324294633No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA42870.10607421483554413No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42220.10446590507013469No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA41920.10372360825533031No Hit
CTATGGTACTAGTGGTTATCACTACTGGGGCCAGGGAACCCTGGTCACCG41800.10342668952940856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)