FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005107797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185921
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGA52910.24204900360077056No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG52220.23889243938824872No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42130.19273340619354495No Hit
GTCTATTACTGTGCGAGAGATTGTGGTGGTGGTGACTTCATTATTTTGGG40380.18472762739367068No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG39410.18029013857316892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.17265033823271747No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG31210.14277734648232943No Hit
GTCTACATGGAGCTGAGCAGCCTGAAATCTGAGGACACGGCCGTCTATTA29490.1349088096047387No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29070.13298742269276886No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACATGAATGCCA26390.12072714430210424No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26350.12054415507239283No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT25740.11775356931929379No Hit
ATTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGGAGTGC24230.11084572589768799No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23700.10842111860401177No Hit
GGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT23380.10695720476632048No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23240.10631674246233053No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG22710.10389213516865431No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22280.10192500094925662No Hit
GATATGAATACCTCCCAAAATAATGAAGTCACCACCACCACAATCTCTCG22060.10091856018584386No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA21930.10032384518928177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)