FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005107806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735121
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAAC100590.36777166348399215No Hit
TGATTATACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGT97380.3560354368234531No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT92750.33910748372741095No Hit
GTATTAAAGTGTTGACAGTAATAAGTTGCCAAATCTTCAGGCTGCAGGGA73840.2699697746461674No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68640.2509578186851697No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT59250.21662661359406038No Hit
ACTTATTACTGTCAACACTTTAATACTTACCCGCGCTTCGGCCAAGGGAC54720.20006427503572966No Hit
ATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACAGGG53060.19399507370971888No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCCTATGCAGACTCTGTGAAG51230.18730432766959854No Hit
CAATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATA50130.1832825677547721No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG49860.18229540850295106No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA46020.16825581025482966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45810.16748801972563554No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA43410.1587132708205597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43340.15845734064416164No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA43050.1573970584847983No Hit
ATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACA42900.15684863667823104No Hit
GTATTATTGTGCAAAAGATAAGGGCAGTGGCTGGTACCGCTGGTTCTTCG41830.15293656112471807No Hit
ACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGTGGGTCTC41410.1514009800663298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41220.15070631244467797No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG40720.14887823975612047No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39610.1448199183875229No Hit
GTGGTAGCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC38910.14226061662354245No Hit
TATTTAGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGAT37500.13710545164181037No Hit
GTAATAAGTTGCCAAATCTTCAGGCTGCAGGGAGCTGATTGTGAGAGTGA37340.13652046838147197No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC37280.13630109965884507No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC36340.13286432300435705No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35650.13034158269414772No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35560.13001252961020737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35050.12814789546787875No Hit
GTCTGAGATCTGAGGACACCGCCTTGTATTATTGTGCAAAAGATAAGGGC34820.12730698203114232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34420.12584452388029635No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG33650.12302929193991784No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT33000.12065279744479313No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT32990.12061623599102196No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA32900.12028718290708162No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA32310.1181300571345838No Hit
ATCTCAGACTGTTCATTTGCAGATACAGGGAGTTATTGTTGTTGTCTCTG31770.11615573863094172No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC31720.11597293136208599No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31500.1151685793791207No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT30720.11231678598497105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA30610.1119146099934884No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT29420.10756379699472163No Hit
ATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT29300.10712505954946783No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA29050.10621102320518909No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT29050.10621102320518909No Hit
ATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACA28840.10544323267599495No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28580.10449263487794506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC28350.10365172144120864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27530.10065368223197439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)