FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799751
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTTCCTTCACTTTCGGCCCTGGGACCAAAGTGGATA224960.5920387941209833No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT140900.37081377174451696No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT132180.3478648995684191No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA119740.31512591219793085No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC110330.2903611315583574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100580.2647015554440278No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA97310.2560957283779911No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTTCCTTCACTTTCGGCCCTG85620.22533055455475898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG71110.18714384179384386No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67270.1770379164318925No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC66670.1754588655940876No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT65830.17324819442116074No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT64820.17059012551085584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63450.166984626097868No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTG62370.16414233458981917No Hit
GTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCT60120.1582208939480508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG59270.15598390526116054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC57250.15066776744055072No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT56410.14845709626762385No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT55640.14643064769244088No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC54770.1441410239776238No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT53750.14145663755335547No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA53640.1411671448997579No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51070.13440354381116024No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC51040.13432459126927002No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC50020.1316402048450017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.1291926760464041No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG49050.12908740599055044No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT48000.12632406702439186No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA47310.12450815856091624No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT46340.12195535970646498No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA45740.12037630886866009No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTTCCTTCACTTTCGGCCC45080.1186393529470747No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA43810.11529702867372098No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA43810.11529702867372098No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTAGCCTGGTACCAGC43710.11503385353408684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43120.11348112021024535No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC42930.11298108744494048No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA42900.11290213490305022No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG42390.11155994169091607No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA42190.11103359141164777No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG40570.10677015414957453No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA40270.10598062873067209No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40200.10579640613292819No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39510.10398049766945254No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC39430.10376995755774522No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38900.10237512931768425No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG38050.10013814063079397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)