FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867197
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTG119520.309061058953035No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC116680.30171723860977345No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG110470.28565909623947267No Hit
ATGTATTACTGTGCGAGACCACGGGGGCTACGGGCCAATCCCTACTACTT107890.27898759747693225No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGC103220.2669116675462874No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT94270.2437682900560794No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC92420.2389844634240252No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT88030.22763257211877233No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG85580.22129723414659247No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT84750.21915097679275197No Hit
GTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG84640.21886653304706224No Hit
CCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA82510.2133586677896161No Hit
AGCCAGGACAGGCCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCC82400.21307422404392637No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG76800.19859345153608673No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA75370.19489568284212053No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA75340.19481810727511426No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGCTGTGTTCGG72320.187008833529815No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA71830.18574176593537906No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT67960.1757345177915684No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT66350.1715712956955645No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAGGGAGACAGCC65090.1683131218813006No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGCTGTGTTCGGAG64850.1676925173452503No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCT64080.1657014111254224No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCA64050.1656238355584161No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA61420.15882304418419851No Hit
GCATAATAGCTTCTGAGGCTGTCTCCCTGGCATGTGATCCTGACTGTCTG60880.15742668397808543No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACAC60580.15665092830802257No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA60200.15566830445927632No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58660.1516860920196204No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT58600.15153094088560784No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG57790.14943640057643817No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC57450.14855721081703363No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC57350.14829862559367935No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG56260.1454800466591177No Hit
GTCAGGATCACATGCCAGGGAGACAGCCTCAGAAGCTATTATGCAAGCTG55640.14387681827432117No Hit
GTATTACTGTGCGAGACCACGGGGGCTACGGGCCAATCCCTACTACTTTG55130.14255803363521435No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT53770.13904127459759616No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA53000.1370501683777682No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT52900.1367915831544139No Hit
GACAAGTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAAT52590.13598996896201565No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG52090.13469704284524425No Hit
GTCTGGAGCAGAGGTGAAAAAGCCGGGGGAGTCTCTGAAGATCTCCTGTA51600.13342997525080827No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG51580.1333782582061374No Hit
GTCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAA51020.13193018095535347No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT49620.12830998782839353No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT49500.12799968556036842No Hit
CATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCC49410.12776695885934955No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48410.12518110662580675No Hit
CCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTG46350.1198542510247086No Hit
CTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGG46170.11938879762267089No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGG45930.11876819308662062No Hit
GTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCC45270.11706153061248237No Hit
GGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAG44800.11584618006271725No Hit
TCCGAACACAGCATGGTCACCACTGCTGTCCCGGGAGTTACAGTAATAGT44720.11563931188403383No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG43920.1135706300971996No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGT43580.11269144033779505No Hit
CACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGG43550.11261386477078876No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG43330.11204497727940935No Hit
TCCTTGACCATCACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTG42960.11108821195299852No Hit
GGTCACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC42750.11054518298395453No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAG42530.10997629549257511No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA42300.10938154947886027No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGG42220.10917468130017685No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG42170.1090453886884997No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGT41420.1071059995133426No Hit
CAGCATGGTCACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCA40980.10596822453058378No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA40880.1057096393072295No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC40730.10532176147219807No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC40680.10519246886052094No Hit
TCTCCAAGGAGTCTGTGCCGAGGTGCAGCTGGTGCAGTCTGGAGCAGAGG39710.10268419219398442No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA39640.10250318253763643No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCT39550.10227045583661758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.10175328538990902No Hit
GATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGGCGACAAGTCC38700.10007248143810621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)