FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577562
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT86770.24253947241165913No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA77970.21794171561527095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC72520.20270787759932604No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG70550.1972013343164982No Hit
GTATATTACTGTGCGAGCGATTATTATGATAGAAGTGCTCATTTCTCCTC58140.16251290683431902No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT53310.14901209259266507No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG52740.14741882880017174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48650.13598646228912314No Hit
GTATGGTAACTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA47670.13324716664588904No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC46560.13014449504998096No Hit
GTTTTACTGTCAGCAGTATGGTAACTCACCGTGGACGTTCGGCCAAGGGA44890.12547651165793913No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT44040.12310059196737891No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43190.12072467227681868No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT40170.11228316937624003No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG39180.10951592173664634No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG36450.10188502673049411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)