FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204258
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT173570.4128433602314606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC148610.3534749770351867No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG93660.22277414944563345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT87390.20786069741676177No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG85080.2023662677219143No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTACTAC82690.1966815547475916No Hit
GAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA80140.19061627521431845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC70540.16778228167729003No Hit
AGTCAGAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAA70100.16673572364017622No Hit
GTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGACCA68880.16383390362817887No Hit
CATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTA68880.16383390362817887No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATC68620.16321548296988436No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT65750.15638907031871022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG63000.14984808258674895No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA61750.14687490634494837No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG59620.1418086140289202No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT59380.1412377641904945No Hit
ACTTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGG58830.13992956664410225No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG57450.1366471800731544No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55630.13231823546509278No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC55280.1314857461173886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT54930.13065325676968445No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA54780.13029647562066837No Hit
CAGTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGAC54640.1299634798815867No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC54530.12970184037230825No Hit
GAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCC53290.1267524495404421No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA53220.12658595167090125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC52960.12596753101260674No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA52510.12489718756555854No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT51990.12366034624896949No Hit
GTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGGGA50880.12102016574625059No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT50350.11975953901972715No Hit
CTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG50280.11959304115018632No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC50210.11942654328064549No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGATTTCTGCTGATACCAATT50030.11899840590182618No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG49790.11842755606340047No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49680.11816591655412204No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48890.11628686916930409No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC48500.11535923818186229No Hit
TCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGT47860.11383697194606039No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47460.11288555554868421No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG47320.11255255980960255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46730.11114922062347267No Hit
TCCATACCGTATGTACGATAACTCCCCCAAAAGTAATCATCTAAAATCTC46290.11010266258635888No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG45150.10739112585383674No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA44620.10613049912731332No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG44510.10586885961803486No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44110.10491744322065867No Hit
AGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGA43950.10453687666170819No Hit
TCTCTAGAGTCCGTGCACAGTAATACGTGGCTGTGTCCTCAGGGTCCATG43770.10410873928288893No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC43710.10396602682328249No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT43610.10372817272393846No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC43340.10308596665570952No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43340.10308596665570952No Hit
TTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAG42650.1014447733702356No Hit
CTCTAGAGATTTTAGATGATTACTTTTGGGGGAGTTATCGTACATACGGT42630.10139720255036679No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC42460.10099285058148191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)