FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751754
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCGACGTTCGGCCAAGGGACCAACGTGGAAATCAAAC161410.43022543588945333No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT135290.36060466651065076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC114340.30476411832971995No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA97220.25913212859904994No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC86970.23181157399978786No Hit
TTGTATTACTGTGCAAAAGGCGGGGGAGCTACTATTACCTTTGACTACTG81780.21797804440269805No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGACCA80930.21571243743592994No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC73690.19641479691898775No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG71780.19132384479366185No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT70390.18761891104800582No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT69590.18548657507928293No Hit
CATCTATGGTGCATCTAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTG68510.182607921521507No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67680.180395622953957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61380.1636034772002642No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT60120.16024504804952563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57100.1521954797675967No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT55090.14683798564618042No Hit
CTCTTATTAGTTGGGACGGTGGTAGCACCTACTATGCAGACTCTGTGAAG54120.1442525282841039No Hit
GTGGTAGCACCTACTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCC53110.14156045412359125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG52430.1397479685501768No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA51560.13742905318419066No Hit
ACTATTACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC51000.13593641800608464No Hit
TAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGTCTGGAGT47400.1263409061468316No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG45360.1209034494265882No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA45350.12087679522697915No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGAC45020.11999720663988098No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC44760.11930419745004603No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC44650.11901100125434663No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGA44290.11805145006842134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44230.11789152487076712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44170.1177315996731129No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT43860.11690531948523278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43350.11554595530517192No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGC43130.11495956291377313No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG42980.11455974991963758No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG41290.11005519018571049No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC41230.10989526498805625No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT41110.10957541459274782No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT41040.10938883519548456No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCC40710.10850924660838637No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40570.10813608781385987No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA40290.10738977022480686No Hit
GCATCTAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTC40050.10675006943418998No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39950.1064835274380996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39640.1056572472502195No Hit
CCTTTAATAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGT39600.10555063045178335No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA39090.10419126627172251No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA39060.10411130367289541No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38970.10387141587641407No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT38400.10235212649869901No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37870.10093945391942008No Hit
TTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCT37660.10037971572763034No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA37520.10000655693310383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)