FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751754
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCGACGTTCGGCCAAGGGACCAACGTGGAAATCAAAC179570.478629462379463No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT131950.35170216384123265No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGACCA96350.25681321323306383No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA91830.24476551500977944No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC90940.2423932912445752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC87660.23365071377281135No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT77510.20659670116963957No Hit
TTGTATTACTGTGCAAAAGGCGGGGGAGCTACTATTACCTTTGACTACTG74510.19860044128692875No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC66890.17828994118484315No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66520.1773037357993088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66390.17695723120439133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63420.1690409339205076No Hit
CATCTATGGTGCATCTAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTG62920.16770822394005577No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT57150.15232875076564187No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG55030.14667806044852622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54780.1460117054583003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53660.14302643510208826No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT51620.13758897838184486No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50730.13521675461664065No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT50650.13500352101976837No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT50160.1336974652389256No Hit
ACTATTACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC49810.1327645682526093No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCGACGTTCGGCCAAGGGAC49370.13159178346981174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49080.13081881168114967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48880.13028572768896896No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC48600.12953941009991593No Hit
CTCTTATTAGTTGGGACGGTGGTAGCACCTACTATGCAGACTCTGTGAAG48270.12865982151281774No Hit
GTGGTAGCACCTACTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCC48140.12831331691790027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC47730.12722049473392977No Hit
TAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGTCTGGAGT47040.12538135496090627No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA46050.1227425891996117No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT45370.12093010362619724No Hit
TTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCT45120.12026374863597132No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA43200.1151461423110364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT42870.11426655372393818No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA42640.11365350713293035No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGC41460.1105083115790641No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA41110.10957541459274782No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41070.10946879779431166No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA40960.10917560159861227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCC40600.10821605041268698No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG39280.1046976960642942No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39140.10432453726976769No Hit
GTTAGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCT38950.10381810747719598No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA38680.10309844408775203No Hit
CCTTTAATAATTATGCCATGCACTGGGTCCGTCAACCTCCGGGGAAGAGT38060.10144588371199179No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37820.10080618292137491No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG37700.10048633252606647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37590.10019313633036707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)