FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543615
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCGAGAGGCGGGCATAACTGGAACGACAAAGGCTACAT58270.22908341081492287No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTACG54970.21610974931347707No Hit
ATGCTATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATG44950.17671699529999627No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAGCAATTTCGATGTGGAATT39700.1560770792749689No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG39290.15446520011872866No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA38780.15246017970486886No Hit
GCTATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGG37480.14734934335581445No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36960.1453050088161927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.13586961863332306No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA33430.13142712242222193No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG31860.1252548046775947No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31490.12380018202440227No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA30880.12140202035292291No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT30090.11829620441772833No Hit
ATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGGATG29400.11558352974015329No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29120.11448273421881848No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28920.11369645170357937No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28810.11326399632019782No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28290.11121966178057607No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27640.10866424360604889No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27130.10665922319218907No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26780.10528322879052059No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG26290.10335683662818469No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26220.103081637747851No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26190.10296369537056511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)