FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4642229
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAGGGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATACA133880.288395940829287No Hit
ATACAGGGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATACAGTG120790.2601982797488017No Hit
GTATTACTGCGCGAGAGTCCGCGTCTCATCTCAATTTGCTGGTTCCGATT107910.23245298756265578No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA102660.22114376520417242No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC101520.21868804834918742No Hit
CTGTATTACTGCGCGAGAGTCCGCGTCTCATCTCAATTTGCTGGTTCCGA97790.210653115130684No Hit
GCCCTGTATTACTGCGCGAGAGTCCGCGTCTCATCTCAATTTGCTGGTTC92780.19986088579430267No Hit
GATAATAAGCGGCCCTCAGGAATTCCTGAGCGATACTCTGGCTCCAACTC92630.1995377651554889No Hit
CTGTAGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCACCACTGTG90750.1954879864823558No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAATTGGTGGAGTCGGGGGGAGGCT89590.19298918687552896No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT85440.18404951586834686No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT84220.18142146800599454No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCGTCA79730.17174939021750113No Hit
CTCCAGGGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA77370.1666656255001638No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT76930.1657178049596433No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC76490.1647699844191228No Hit
CATCTACAGAGAGGGTCTCGCTGATGGTCAGAGTGGCTGTGTCCCCAGAG75240.1620773124290077No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATCAGT75220.16203422967716585No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG74200.15983700933323194No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA74200.15983700933323194No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT73450.15822140613916288No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT71780.1546239963603691No Hit
TCTATGAAGATAATAAGCGGCCCTCAGGAATTCCTGAGCGATACTCTGGC69750.1502510970484222No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC67940.14635210800673554No Hit
ATTCAGAAGAATGTCCACAAATTACGCAGACCCTGTGAAGGGCCGCTTCA66870.14404718078319703No Hit
CTATTACTGTCAGGCGTGGGACACCACCACTGTGATCTTCGGCGGCGGGA66690.14365943601662046No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG66340.14290548785938825No Hit
CAGTAATACAGGGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATA66320.14286240510754641No Hit
CGTATGAACTGACTCAGTCCCCCTCAGTGTCCGTGTCCCCAGGACAGACA65970.14210845695031415No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG64790.13956657459164554No Hit
CTTCTGGTCATCTATGAAGATAATAAGCGGCCCTCAGGAATTCCTGAGCG63740.13730473011994884No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG63730.13728318874402792No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA63240.1362276613239028No Hit
CTTATTATCTTCATAGATGACCAGAAGAGGGGACTGGCCTGTTTTCTGCT62180.1339442754762852No Hit
GTAATAGTCAGCCTCATCTACAGAGAGGGTCTCGCTGATGGTCAGAGTGG61420.13230713090629523No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA60510.13034686569749146No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC59700.12860201424789686No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA59460.12808502122579477No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC59380.12791269021842738No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG59050.12720182481303702No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC58810.12668483179093493No Hit
GTCCCTGGAGATGGTGAAGCGGCCCTTCACAGGGTCTGCGTAATTTGTGG58500.12601704913738637No Hit
GACTATTACTGTCAGGCGTGGGACACCACCACTGTGATCTTCGGCGGCGG58250.12547851473936336No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC58210.12539234923567968No Hit
CTCTTGTGAAGCCTCTGGATTCACCTTCGGTGACTTCTACATGAGTTGGG58020.12498306309318218No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG57780.12446607007108008No Hit
GTCTTCTCCACGGTGCTCCCGTCATGCGTGACCTGGCAGCTGTAGCTTCT56760.12226884972714616No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG55750.12009317075913316No Hit
CTTCTACATGAGTTGGGTGCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG55680.11994238112768671No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC54460.11731433326533439No Hit
CATTGGTAGTCCATTCAGAAGAATGTCCACAAATTACGCAGACCCTGTGA54320.11701275400244149No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCGTCATGCGTGACCTGG53800.11589260245455361No Hit
GGGGATAAATTGGCCAATAAATTTGTTTGCTGGTATCAGCAGAAAACAGG53320.11485861641034943No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT52600.11330763734404313No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT52280.11261831331457366No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC51750.11147662039076486No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC51320.11055034122616529No Hit
CCCCTCTTCTGGTCATCTATGAAGATAATAAGCGGCCCTCAGGAATTCCT51300.11050725847432344No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51190.11027030333919331No Hit
CAGTAATAGTCAGCCTCATCTACAGAGAGGGTCTCGCTGATGGTCAGAGT50840.10951635518196108No Hit
GTCCTTGACCAGGCAGCCCAGGGCGGCTGTGCTCTCGGAGGTGCTCCTGG50370.1085039105136778No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA49940.1075776313490782No Hit
CCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGT49320.10624206604198112No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA49160.1058974040272464No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC48690.10488495935896311No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCGTCATGCGTGACC48540.10456183872014929No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC48390.1042387180813355No Hit
GAACACAGAGGACTCACCATGGAGTTTGGACTGAGATGGGTTTTCCTTGT48190.10380789056291709No Hit
CTTCTGAATGGACTACCAATGTATGCGACCCACTCCAGCCCCTTCCCTGG48120.10365710093147064No Hit
CTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCCCTG47670.10268773901502921No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT47550.10242924250397815No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG47370.10204149773740158No Hit
TTGGTAGTCCATTCAGAAGAATGTCCACAAATTACGCAGACCCTGTGAAG47000.10124446682832752No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46660.10051206004701621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)