FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658967
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAAC126900.3468191978774337No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT101510.27742802818391094No Hit
GTATTAAAGTGTTGACAGTAATAAGTTGCCAAATCTTCAGGCTGCAGGGA91470.24998858967571996No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91430.24987926920357578No Hit
TGATTATACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGT88730.24250013733384312No Hit
ACTTATTACTGTCAACACTTTAATACTTACCCGCGCTTCGGCCAAGGGAC63970.1748307650765913No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA62220.17004799442028312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61700.1686268282824087No Hit
ATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACAGGG58300.15933458815015278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57950.1583780340188911No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG55320.15119021297541083No Hit
CAATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATA55220.15091691179505037No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55130.15067094073272594No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCCTATGCAGACTCTGTGAAG54140.1479652590471573No Hit
GTATTATTGTGCAAAAGATAAGGGCAGTGGCTGGTACCGCTGGTTCTTCG53770.14695404467982356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53330.14575151948623752No Hit
TATTTAGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGAT52700.14402972204996659No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51090.13962957304616302No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA50440.13785311537381997No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT50230.137279182895063No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG49420.13506544333414322No Hit
ATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACA48570.13274238330107924No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG47010.12847888488745596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46000.12571854296581522No Hit
GTAATAAGTTGCCAAATCTTCAGGCTGCAGGGAGCTGATTGTGAGAGTGA45420.12413339611972449No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT43880.11992455794217328No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC43540.1189953339289477No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT42730.11678159436802792No Hit
GTGGTAGCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC41900.11451319457103602No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40740.11134290087885461No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39940.10915649143597085No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA39400.10768066506202432No Hit
ACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGTGGGTCTC39170.10705207234719527No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC38690.105740226681465No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA38650.10563090620932082No Hit
GTCTGAGATCTGAGGACACCGCCTTGTATTATTGTGCAAAAGATAAGGGC38450.1050843038485999No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37690.10300721487786033No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37410.10224197157285102No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG37350.10207799086463475No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37030.10120342708748126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36840.10068415484479636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)