FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4764678
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT169820.35641443136346257No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC142670.29943261643284186No Hit
ATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA123590.2593879376528697No Hit
GTCTCTGGTGGCTCCGTCAATAGTAATAGAACTAATATTTGTTACTGGGC117030.245619955850112No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC100240.21038147803482207No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA99600.20903826029796765No Hit
ACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACACGTCC97250.20410613267045538No Hit
ATTTATTATTGTGCGAGAGCGTATTATTTTAGTGATTTCGAGTGGTATTT96660.20286785381929273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG89600.1880504831596175No Hit
TTATTATTGTGCGAGAGCGTATTATTTTAGTGATTTCGAGTGGTATTTTG87230.1830763799778285No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT86060.18062081005264155No Hit
CATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTG85260.17894178788157353No Hit
ATTATGATGGGAGCACCTACTACAACCCGTCCCTCAAGACTCGAGTCACC83020.1742405258025831No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC81810.1717010047688427No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT80980.16995901926635967No Hit
CAATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGA79790.16746147378689596No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT79660.16718863268409745No Hit
ATCTATTATGATGGGAGCACCTACTACAACCCGTCCCTCAAGACTCGAGT79080.16597134161007313No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT74890.1571774629891044No Hit
GAGTTACAATCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAACCA72000.15111199539612122No Hit
GATTACACTCTCACCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTA70850.14869840102521092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC70670.14832062103672064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG67030.14068107015836118No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT66370.13929587686723005No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA66030.13858229244452616No Hit
AGTCAGAACATTACCAACTTTTTAATTTGGTATCAAAAAAAACCAGGGAA64830.13606375918792413No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGGGTCACCATCACTT63370.13299954372572503No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG63220.13268472706864975No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC62320.13079582712619825No Hit
ACTTACTACTGTCAACAGAGTTACAATCTCCCGTGGACGTTCGGCCAAGG62290.1307328637947832No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG61470.12901186606943849No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG61190.1284242083095647No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA60640.1272698805669554No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA60220.1263883939271447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC58360.12248466737941156No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC58220.12219083849947467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT58100.12193898517381449No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCC57860.12143527852249408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG57550.12078465743120521No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT57130.11990317079139451No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG57020.11967230524287265No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG56610.1188118063802003No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC56250.1180562464032197No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCGCCGAAGGCTTCACCAGTC56120.11778340530042115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC55270.11599944424366138No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA54650.11469820206108365No Hit
CTGTAGATGGTGAGAGTGTAATCTGACCCAGACACACCGCCACCGAACCT54430.11423647096403997No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG54360.11408955652407152No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC52360.10989200109640149No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG52010.10915742889655922No Hit
CACCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC51940.10901051445659077No Hit
GTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCCCTTGGCCGAACGTC51320.10770927227401306No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA51310.1076882844968747No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG51170.10739445561693779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.10733149228552274No Hit
CTATTATGATGGGAGCACCTACTACAACCCGTCCCTCAAGACTCGAGTCA51100.10724754117696934No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC50810.10663889563995721No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50180.10531666568024114No Hit
TCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTGGC50130.10521172679454939No Hit
ACCTACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACAC50120.10519073901741104No Hit
ATAATAGATAGTCGCAAGCCACTCCAGCCCCTTTCCGGGGGACTGGCGGA49970.10487592236033579No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG49900.10472900792036734No Hit
GACCTACACCTGCAATGTAGATCACAAGCCCAGCAACACCAAGGTGGACA48880.10258825465225563No Hit
CCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG48820.10246232798942552No Hit
ATTTTAGTGATTTCGAGTGGTATTTTGACTACTGGGGCCAGGGAAACCTG48120.100993183589741No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48030.10080429359549585No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC47880.1004894769384206No Hit
GTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAA47800.1003215747213138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)