FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4764678
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT166470.34938352602211525No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT148210.31105984496748784No Hit
ATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA124640.2615916542523965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC114880.24110758376536673No Hit
GTCTCTGGTGGCTCCGTCAATAGTAATAGAACTAATATTTGTTACTGGGC110920.23279642401858006No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA100220.2103395024805454No Hit
CATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTG99810.20947900361787303No Hit
ACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACACGTCC91160.19132457639320014No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC89440.1877146787254039No Hit
ATTTATTATTGTGCGAGAGCGTATTATTTTAGTGATTTCGAGTGGTATTT84910.17820721568173128No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT84590.17753560681330408No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG81770.17161705366028931No Hit
TTATTATTGTGCGAGAGCGTATTATTTTAGTGATTTCGAGTGGTATTTTG78700.16517380607881582No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC78660.1650898549702624No Hit
ATTATGATGGGAGCACCTACTACAACCCGTCCCTCAAGACTCGAGTCACC77360.1623614439422769No Hit
ATCTATTATGATGGGAGCACCTACTACAACCCGTCCCTCAAGACTCGAGT76170.1598638984628132No Hit
CAATAATAAATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGA75510.1584787051716821No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT74970.1573453652062112No Hit
GAGTTACAATCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAACCA72470.15209842092162368No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA72000.15111199539612122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG71090.14920210767653133No Hit
GATTACACTCTCACCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTA69720.14632678220857737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67440.14154156902103351No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT66190.13891809687873977No Hit
ACTTACTACTGTCAACAGAGTTACAATCTCCCGTGGACGTTCGGCCAAGG65310.13707117249056494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63030.13228595930302112No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC61980.13008224270349433No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGGGTCACCATCACTT61430.1289279149608851No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA61240.12852914719525643No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA60920.12785753832682922No Hit
AGTCAGAACATTACCAACTTTTTAATTTGGTATCAAAAAAAACCAGGGAA60710.12741679500692388No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC58980.12378590956198929No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT57620.12093157187117368No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCGCCGAAGGCTTCACCAGTC57550.12078465743120521No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG56850.1193155130315207No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC56110.1177624175232828No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA56070.1176784664147294No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAACTGCT55670.11683895532919539No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT55030.11549573759234097No Hit
TCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTGGC54110.11356486209561276No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA53210.11167596215316124No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCC53090.11142410882750103No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT52880.11098336550759567No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT52600.1103957077477219No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA52580.11035373219344519No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC52530.11024879330775343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52300.10976607443357138No Hit
CTGTAGATGGTGAGAGTGTAATCTGACCCAGACACACCGCCACCGAACCT51780.10867471002237716No Hit
CACCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC51060.10716359006841594No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG51050.10714260229127762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50110.1051697512402727No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA49820.10456110570326053No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA49770.10445616681756878No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC49220.10330183907495953No Hit
ACCTACTACAACCCGTCCCTCAAGACTCGAGTCACCATGTCAGTTGACAC49170.10319690018926779No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG48930.10269319353794738No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC48710.10223146244090367No Hit
CCATCTACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG48690.10218948688662698No Hit
TGAGAAGACATTCCCCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGC48670.10214751133235027No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT48210.10118207358398616No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG48010.10076231804121916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)