FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005107942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977040
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT193210.3882026264607076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC129640.2604761062800379No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG99040.19899377943516627No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT95880.19264462411393118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG91100.18304052207737934No Hit
GAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA89150.17912253066079437No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTACTAC87400.1756063845177053No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT76710.15412775464934983No Hit
AGTCAGAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAA74080.1488434893028788No Hit
CATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTA73990.14866265892980568No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG73140.14695481651744813No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATC72930.14653287898027742No Hit
GTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGACCA71980.14462411393117194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC71940.14454374487647276No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT70460.14157008985260316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68760.13815440502788806No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG68750.13813431276421326No Hit
ACTTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGG65910.13242810988057158No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA65750.13210663366177489No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT63890.12836947261826306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT63180.12694292189735265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC62160.12489351100252359No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC61860.12429074309227976No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA60210.12097551958593863No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA59580.11970970697442658No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA58460.11745937344284957No Hit
CAGTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGAC57530.11559079292109366No Hit
GAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCC57160.11484737916512627No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA56440.11340073618054106No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC54710.10992477456480156No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGATTTCTGCTGATACCAATT54360.10922154533618375No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG53920.1083374857344928No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT52790.106067059939241No Hit
TCCATACCGTATGTACGATAACTCCCCCAAAAGTAATCATCTAAAATCTC52560.10560493787472071No Hit
GTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGGGA52370.10522318486489962No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC52340.10516290807387524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG51760.10399755678073715No Hit
TCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGT51020.10251072926880235No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA50700.1018677768312089No Hit
CCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGA50660.10178740777650973No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG50400.10126500892096507No Hit
TCTCTAGAGTCCGTGCACAGTAATACGTGGCTGTGTCCTCAGGGTCCATG49880.10022021120987576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49800.10005947310047739No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)