FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537124
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATGGCGGTGTCGGAGTCCCTCAGACTACTCCACTGCAAATATG158780.34995737387825415No Hit
ATCTATTACTGTGCGCGAGTCCCCGCCAGCAGCTGGTCCGTGGGCTACTT146470.3228256490234783No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT117220.25835749695181354No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT112350.24762382513680475No Hit
AGTTTACACTGGTATCAACAGAAAGCAGATCAGTCGCCAAAGATCTTCGT108230.238543182862095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86130.1898339124079483No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC83610.18427973315254334No Hit
ACATATTACTGTCAACAGACTTATATTTTACCATTCACTTTCGGCCCTGG81310.17921044256229277No Hit
GACTTATATTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGGTATCA73990.16307687424897357No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA73340.16164424864738103No Hit
ATATAAGTCTGTTGACAGTAATATGTTGCAGCATCTTCAGCTTCCAGGCT71230.1569937255406729No Hit
ATATTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGA65920.1452902763953553No Hit
CAGTAATAGATGGCGGTGTCGGAGTCCCTCAGACTACTCCACTGCAAATA63120.139118966111572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61880.13638595727161082No Hit
ATATTACTGTCAACAGACTTATATTTTACCATTCACTTTCGGCCCTGGGA59570.1312946262874896No Hit
GTAATATGTTGCAGCATCTTCAGCTTCCAGGCTAGTGATGGTGAGGGTGA59050.13014852580621555No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA55570.12247846873922777No Hit
GGTATCAACAGAAAGCAGATCAGTCGCCAAAGATCTTCGTCAAGTATGCT55470.1222580648005212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55220.12170705495375485No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC55210.1216850145598842No Hit
CTATTACTGTGCGCGAGTCCCCGCCAGCAGCTGGTCCGTGGGCTACTTTG54890.12097972195602325No Hit
GTCTGGAGCAGAGGTGAGAAAGCCCGGGGAGTCCCTGAAGATCTCCTGTA52210.11507289639868781No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50240.11073093880616884No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT48500.10689591027267493No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT46920.10341352804111152No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC46400.10226742755983748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)