FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005107968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5148750
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT206070.40023306627822286No Hit
ATAATACACAGCCGTGTCTTCAGGTCTCAGGCTGCTCAACTCCATGTAGG164420.31933964554503524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC161390.31345472201990776No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT150300.2919155134741442No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT140390.27266812333090557No Hit
GTGTATTATTGTGCGAGATGCTCCGATTTTTGGAGTGGTTATGAGTGGGC124010.24085457635348384No Hit
CTCATAACCACTCCAAAAATCGGAGCATCTCGCACAATAATACACAGCCG121800.23656227239621266No Hit
GTATTATTGTGCGAGATGCTCCGATTTTTGGAGTGGTTATGAGTGGGCGG117750.2286962855061908No Hit
GTCTGGGGGTGAGGTGAAGAAGCCTGGGGCCTCTGTGAAGGTTTCCTGTA113650.22073318766690944No Hit
GAGTTACACTACCCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCA113030.21952901189609125No Hit
GGTTATGAGTGGGCGGAGGCTTTTGATATCTGGGGCCAAGGGACAACAGT110090.21381888807963095No Hit
CTCCTGATCTATGCTGCATCCAGCTTGCAAAGTGGGGTCCCATCAAGGTT104330.20263170672493325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT98710.19171643602816216No Hit
ACACTACCCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA96230.18689973294488954No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA95210.18491866957999514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG90820.1763923282350085No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG89270.1733818888079631No Hit
CAATAATACACAGCCGTGTCTTCAGGTCTCAGGCTGCTCAACTCCATGTA86420.16784656470016995No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC82980.16116533139111433No Hit
ATGCTGCATCCAGCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT82930.16106822044185481No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC81080.15747511531925223No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT81040.15739742655984462No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA80780.15689244962369508No Hit
GTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACT80730.15679533867443554No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA78300.1520757465404224No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT78050.15159019179412478No Hit
GATCTATGCTGCATCCAGCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG76360.1483078417091527No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG76200.1479970866715222No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT75460.1465598446224812No Hit
GTAGGTGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCAA74210.14413207089099295No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC72380.1405778101480942No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA70980.13785870356882737No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG69490.13496479728089342No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT69230.13445982034474385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67880.13183782471473657No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC67550.13119689244962368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC66250.12867200776887594No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG66020.1282252974022821No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG65670.12754552075746542No Hit
CTACTGATAGTATCCATTGGGTGCGCCAGTCTGCCGGACAAAGGCTTGAG64060.12441854819130857No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT63850.12401068220441855No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC62020.12045642146151977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA61940.12030104394270454No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGC61260.11898033503277494No Hit
GGGTAACACAGAATATTCACAGAAGTTCCAGGGCAGAGTCTCCTTTACCA59920.11637776159261956No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT59680.11591162903617383No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT59600.11575625151735859No Hit
GTCTGCATCTGTAGGTGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA58840.11428016508861374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT58490.11360038844379704No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT58280.11319252245690702No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTCTCACTTTCGGCCCTGG56760.11024034959941734No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG56680.11008497208060208No Hit
GTGTTACCCCTGCCAACGTTGATCCATCCCATCCACTCAAGCCTTTGTCC56180.1091138625880068No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG55800.10837581937363439No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC55430.10765719834911387No Hit
ATACACAGCCGTGTCTTCAGGTCTCAGGCTGCTCAACTCCATGTAGGCTG55240.10728817674192764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA54940.10670551104637047No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA52890.1027239621267298No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG51960.10091769847050255No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG51530.1000825443068706No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC51530.1000825443068706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)