FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751682
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA159190.335018210393709No Hit
GTATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTG138170.29078124335761524No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG132260.2783435423498458No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTATGTCTT124320.2616336699299322No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT90530.19052200883813353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84890.1786525276733586No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC81150.17078163058891566No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG79630.1675827633246501No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT78960.1661727363068488No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC75010.15785989045563234No Hit
CTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA73230.15411384852774238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.14836851455968644No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA68990.14519069247479105No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTACTTACTACTGGGGCTGGATCCG67640.1423495932598183No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCT66540.14003462352909982No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT66070.1390455000987019No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA62150.13079578978559592No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA61240.1288806784629106No Hit
ATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACT60530.12738647072762865No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC59300.12479791366509796No Hit
ATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTGGG58750.1236404287997387No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA58120.1223145824994181No Hit
ACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG57710.12145173014524122No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA57690.12140963978650088No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC57150.12027320010051179No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC56210.11829495323971595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG54680.11507504079608022No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA54430.114548911311826No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC53950.11353874270205794No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT53140.11183408317307429No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG52310.11008733328535032No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG52200.10985583631227848No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT49500.10417363788233303No Hit
GTCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC48590.10225852655964772No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC47820.10063804774814476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)