FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5065464
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATTAACAATGATAGTCACTACTGGACCTGGATCCG200380.3955807404810299No Hit
TCACTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCTGTGAC120620.2381223121909464No Hit
GATTATTACTGTTGCTCATATGCAGGTAGCACCAATTTCGTCTTCGGCGG106630.21050391435019578No Hit
CTACTGGACCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGATTGGATTG104690.20667405789479504No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC96320.19015039885783416No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG95510.18855133508006375No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA91830.18128645273167474No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA90250.17816729128861641No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT89770.17721969793882653No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCGGCCGTGATGTT89080.17585753249850358No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA87670.17307397703349583No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC86580.170922150468348No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACGAAATTGGTGCTA84940.16768453985656595No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT84350.16651978969744924No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG83300.16444692924478388No Hit
GTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA83130.1641113232667333No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA81370.1606368143175038No Hit
CACCTGCACTGTCTCTGGTGGCTCCATTAACAATGATAGTCACTACTGGA79220.15639238577157No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG78910.1557803983998307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT78300.15457616518447273No Hit
GGATTGGATTGGGCGTATTTCTACCAGTGGGAGCACCAATTACAACCCCT78070.15412211003769843No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC76810.15163467749450002No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC73810.14571221905831333No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG73520.14513971474281526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT73170.1444487612585935No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC73090.1442908290336285No Hit
CCATATCACTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCT72810.1437380662462511No Hit
ATACAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACT72200.14253383303089312No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCT72060.1422574516372044No Hit
ACGTAGGAGTCACCGTACAGTCCCGCCCTCGCACAGAAATACAGGGCCGT68440.13511101845753914No Hit
GTACGGTGACTCCTACGTTCCTCCCTTTGACTTCTGGGGCCAGGGAACCC67640.13353169620788935No Hit
TTATTACTGTTGCTCATATGCAGGTAGCACCAATTTCGTCTTCGGCGGAG66910.13209056465508393No Hit
CTCCTACGTTCCTCCCTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCG65140.12859631417773376No Hit
CTCCTGCACTGGAACCGGCCGTGATGTTGGGAGTTATAACCTTGTCTCCT65110.1285370895933719No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG62710.12379912284442254No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62540.12346351686637197No Hit
GAAATACAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA62370.1231279108883214No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA61310.12103530890753543No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATACTTTTTGAGGGCA61310.12103530890753543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA60990.12040358000767551No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG60670.11977185110781559No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA60650.11973236805157435No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT60580.11959417735472999No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT60190.11882425775802573No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC59360.11718571092401407No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG57960.11442189698712694No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57880.11426396476216197No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA57740.11398758336847326No Hit
GTCTAGTGATATGGTGATTCGACTCTTGAGGGAGGGGTTGTAATTGGTGC57330.11317818071552774No Hit
TCACCGTACAGTCCCGCCCTCGCACAGAAATACAGGGCCGTGTCTGCGGC56950.11242800264694408No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA56240.11102635415037991No Hit
GTATTTCTGTGCGAGGGCGGGACTGTACGGTGACTCCTACGTTCCTCCCT56240.11102635415037991No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA56140.1108289388691737No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA55280.10913116745080018No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT55220.10901271828207644No Hit
GTCGATCACCATCTCCTGCACTGGAACCGGCCGTGATGTTGGGAGTTATA54190.10697934088565234No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC53770.10615019670458618No Hit
GCCCTGTATTTCTGTGCGAGGGCGGGACTGTACGGTGACTCCTACGTTCC53580.10577510767029437No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC52620.10387992097071463No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC52560.1037614718019909No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG52460.10356405652078467No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT51930.10251775553039169No Hit
ATTTCTACCAGTGGGAGCACCAATTACAACCCCTCCCTCAAGAGTCGAAT51690.10204395885549675No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG51210.10109636550570689No Hit
CACTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCTGTGACC50990.1006620518870532No Hit
TTACAACCCCTCCCTCAAGAGTCGAATCACCATATCACTAGACACGTCCA50770.1002277382683995No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)