FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414040
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC301720.6835461391378419No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG238650.5406611630161938No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA203160.4602586292829245No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG180300.4084693387463639No Hit
GTGTATTACTGTGCGAGAGGGGGTAACGGTGGCTACCATGACTACTGGGG158820.3598064358274959No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT137470.3114380476842077No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG125550.2844333082618191No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC110730.2508586238457286No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC105250.23844369330590567No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC88910.20142545151380595No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT87930.19920526320558943No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT87340.19786861922411214No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG83980.1902565450245127No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT80070.18139844677438358No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG79830.18085472718869788No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC76730.1738316825402579No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACT72950.16526809906570852No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGA71590.16218702141348967No Hit
GTATTACTGTGCGAGAGGGGGTAACGGTGGCTACCATGACTACTGGGGCC71160.16121285715580286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71030.1609183423802231No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC70840.16048789770822194No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA70610.15996683310527318No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC67700.15337423312883436No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA65010.1472800427726074No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGGTAGC64900.1470308379625015No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA60880.1379235349022664No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG60730.13758371016121287No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCC59800.13547679676668087No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG59670.1351822819911011No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG58080.13158013973593352No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC58050.1315121747877228No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGA50790.11506465732073112No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC50420.11422642295946571No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT50000.11327491368451578No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT49580.11232340440956584No Hit
CCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTC48430.10971808139482198No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC48300.10942356661924224No Hit
CCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCT46850.10613859412239128No Hit
ACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAG45940.1040769906933331No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT45570.10323875633206768No Hit
GTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTGAAGCCTTCGG45560.10321610134933078No Hit
CCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAA45460.10298955152196175No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT44980.10190211235059038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)