FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80740.478098029636867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.27813059762253706No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG45950.27209071664372103No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAAATACA44060.2608991724770914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.2514248493730663No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGA39410.2333644209560184No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36740.21755414427617653No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA35970.21299462628236446No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC35870.21240248108836288No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA34730.20565202587674497No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTACACTTGGGTTTTCGGCGG34250.20280972894553745No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG32400.19185504285650842No Hit
TTGTAGTAGTATACGATGCTTTTGATATTTGGGGCCAAGGGACAATGGTC32030.1896641056387026No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31670.18753238294029695No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31320.18545987476129147No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG31110.18421636985388817No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30300.17941999378247547No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA29670.17568947906026558No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29630.17545262098266495No Hit
GTGTATTACTGTGCGAGACACCATACCCGACAGGATATTGTAGTAGTATA29330.17367618540066024No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28650.16964959808144958No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTAGTTG27780.16449793489363593No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA27330.16183328152062887No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG26410.15638554573581442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25940.15360246332400704No Hit
CTACTACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACACAGCC25560.1513523115868011No Hit
ACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACACAGCCGTGTC25390.15034566475699843No Hit
GTAGTATACGATGCTTTTGATATTTGGGGCCAAGGGACAATGGTCACCGT24910.14750336782579088No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC24350.1441873547393821No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23970.14193720300217613No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23840.1411674142499741No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA23150.13708161241136327No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGACAATT23010.13625260913976106No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA23000.1361933946203609No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA22870.13542360586815885No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22810.13506831875175793No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT22770.1348314606741573No Hit
GTATACTACTACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACA22670.13423931548015572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22670.13423931548015572No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22610.1338840283637548No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22340.13228523633995057No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22200.13145623306834836No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT22050.13056801527734602No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21870.12950215392814318No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21710.12855472161774067No Hit
GTTATATGGTATGATGGAAGTAATAAATACTATGCAGACTCCGTGAAGGG21700.1284955070983405No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG21630.12808100546253942No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA21520.12742964574913768No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA21270.12594928276413378No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG21190.12547556660893253No Hit
CTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT21060.12470577785673048No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20990.12429127622092938No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20970.12417284718212905No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20710.12263326967772498No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20690.12251484063892466No Hit
GCTCATATGCAGGCAACTACACTTGGGTTTTCGGCGGAGGGACCAAGCTG20650.12227798256132404No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTAGTTGC20100.11902118399431541No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20010.11848825331971401No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT19910.11789610812571241No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG19720.11677103225710944No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA19610.11611967254370771No Hit
GGATATTGTAGTAGTATACGATGCTTTTGATATTTGGGGCCAAGGGACAA19140.11333659013190035No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19110.11315894657369988No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19060.1128628739766991No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT18770.11114565291409453No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT18710.1107903657976936No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG18460.10931000281268967No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18460.10931000281268967No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18010.10664534943968262No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA18000.10658613492028246No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA17830.10557948809047978No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.10421755414427618No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT17540.10386226702787524No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17300.10244111856227148No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17290.10238190404287131No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17120.10137525721306866No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATA16960.10042782490266615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)