FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.46009681573921923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74990.4440496809817767No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGA48670.2881970659205637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.2627940370978964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.25787923198768337No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTACACTTGGGTTTTCGGCGG42240.2501221299462628No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG39600.2344894968246214No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAAATACA37650.22294266554159078No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC36260.214711847344969No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.20334265962013887No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA34070.20174386759633464No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA32690.19357226391911297No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31820.18842060073129932No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31070.18397951177628755No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30700.18178857455848174No Hit
TTGTAGTAGTATACGATGCTTTTGATATTTGGGGCCAAGGGACAATGGTC30420.18013056801527735No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGACAATT30360.1797752808988764No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG29880.17693298396766888No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27650.16372814614143388No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26860.15905019910882148No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26640.15774747968201802No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA26110.15460911015380974No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25990.15389853592100783No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC25940.15360246332400704No Hit
GTATTTATTACTTCCATCATACCATATAACTGCCACCCACTCCAGCCCCT25640.15182602774200235No Hit
GTTATATGGTATGATGGAAGTAATAAATACTATGCAGACTCCGTGAAGGG25580.1514707406256014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25150.14892451629139467No Hit
GTGTATTACTGTGCGAGACACCATACCCGACAGGATATTGTAGTAGTATA24950.1477402259033915No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA24700.14625986291838758No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT24150.14300306435137897No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTAGTTG23610.1398054803037705No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA23580.13962783674557No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG23580.13962783674557No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23360.13832511731876657No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT22920.13571967846515964No Hit
GTAGTATACGATGCTTTTGATATTTGGGGCCAAGGGACAATGGTCACCGT22890.13554203490695918No Hit
CCATATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC21850.12938372488934285No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT21790.12902843777294193No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21730.12867315065654097No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA21710.12855472161774067No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21670.12831786354014005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21450.1270151441133366No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21150.1252387085313319No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20800.12316620035232638No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20580.12186348092552293No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20570.12180426640612278No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG20530.12156740832852216No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG20510.12144897928972184No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20200.11961332918831698No Hit
CTACTACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACACAGCC20190.11955411466891683No Hit
GCTCATATGCAGGCAACTACACTTGGGTTTTCGGCGGAGGGACCAAGCTG20070.11884354043611495No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20010.11848825331971401No Hit
CTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT19950.11813296620331304No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA19940.11807375168391289No Hit
ACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACACAGCCGTGTC19750.1169486758153099No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19530.11564595638850646No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19430.11505381119450489No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19150.1133958046513005No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT18780.11120486743349468No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18620.11025743512309218No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG18450.10925078829328952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA18400.10895471569628873No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18370.10877707213808827No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18220.10788885434708591No Hit
GTATACTACTACAATATCCTGTCGGGTATGGTGTCTCGCACAGTAATACA17960.10634927684268183No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG17960.10634927684268183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG17910.10605320424568106No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC17640.1044544122218768No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17600.10421755414427618No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT17370.10285562019807257No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17320.10255954760107179No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC17290.10238190404287131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)