FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324004
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT56410.24272763730182906No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT40860.17581725332658638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.1603697756114017No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT35130.15116152984246156RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT27450.11811511512028379TruSeq Adapter, Index 11 (95% over 21bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC26990.11613577257182002No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT25390.10925110283803298RNA PCR Primer, Index 39 (96% over 25bp)
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT24100.10370033786516718No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA24010.10331307519264167No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA23450.1009034407858162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)