FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686254
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATTCTAGCTCACCATTCACTTTCGGCCCTGGGA35840.5222556079818842No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT33240.4843687614206985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30070.4381759523441758No Hit
GTCTCTGGTGGCTCCATCAGCACTAGGACTTACTACTGGGGCTGGATCCG29970.4367187659379763No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCGTCAAAAGTCGCGTCA28740.418795373141723No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT23990.34957901884724896No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC20780.3028033352082465No Hit
TCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC19650.2863371288181927No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18740.27307673252177767No Hit
ACTACAACCCGTCCGTCAAAAGTCGCGTCAGCATGTCCGTAGACACGTCG18210.2653536445689205No Hit
CTCTATTATAGTGGGAGCACCTACTACAACCCGTCCGTCAAAAGTCGCGT17230.2510732177881659No Hit
ATAATACACTGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA17120.24947031274134648No Hit
GTATTATTGTGCGAGACATAGTAGTATTGTGGGGAGGGGTGCATTTGATA16950.24699309585080742No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCGTCAAAAGTCGCGTCAGC16870.24582734672584788No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16520.24072719430414977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15680.22848682849207436No Hit
TAGTAGTATTGTGGGGAGGGGTGCATTTGATATCTGGGGCCAAGGGACAA15190.22134661510169706No Hit
CATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG14370.2093976865708615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14200.20692046968032243No Hit
CAATAATACACTGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14030.20444325278978337No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT13780.20080028677428471No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13770.20065456813366478No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13720.19992597493056508No Hit
GTATTCTAGCTCACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA13590.19803163260250578No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13540.19730303939940608No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13520.19701160211816615No Hit
GTAGTATTGTGGGGAGGGGTGCATTTGATATCTGGGGCCAAGGGACAATG12990.18928851416530904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT12660.18447979902485087No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12280.17894249068129295No Hit
TCCGTAGACACGTCGAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGAC12280.17894249068129295No Hit
ATACTACTATGTCTCGCACAATAATACACTGCCGTGTCTGCGGCGGTCAC12160.1771938669938536No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11930.17384233825959483No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11920.1736966196189749No Hit
CTATAATAGAGACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG11860.17282230777525523No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11620.16932506040037654No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11540.16815931127541697No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTCCTTAGCCTGGTACC11420.16641068758797764No Hit
GTGTATTATTGTGCGAGACATAGTAGTATTGTGGGGAGGGGTGCATTTGA11280.16437062661929838No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA11140.16233056565061915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG11050.16101909788503965No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA10540.15358744721342243No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10510.1531502912915626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10470.1525674167290828No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10460.15242169808846287No Hit
GGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGG10440.152130260807223No Hit
CAATACTACTATGTCTCGCACAATAATACACTGCCGTGTCTGCGGCGGTC10260.14950732527606397No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10140.1477587015886246No Hit
GTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC9880.14397001693250605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9840.14338714237002625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9830.14324142372940632No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9720.1416385186825869No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9410.1371212408233686No Hit
ATAATAGAGACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA9350.13624692897964893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9290.13537261713592927No Hit
CACTTGCACTGTCTCTGGTGGCTCCATCAGCACTAGGACTTACTACTGGG8960.13056390199547105No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8950.1304181833548511No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8860.12910671558927161No Hit
GTGTATTATTGTCAGCAGTATTCTAGCTCACCATTCACTTTCGGCCCTGG8840.12881527830803172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8780.12794096646431205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8580.12502659365191313No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8360.12182078355827435No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8260.12036359715207488No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8170.11905212938649538No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA8140.11861497346463555No Hit
CCTCATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCA8130.1184692548240156No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8120.11832353618339567No Hit
GACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGT8100.11803209890215577No Hit
TCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGTTCCCAGACTCCTCAT7950.11584631929285659No Hit
ATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTG7940.11570060065223664No Hit
GTATTGTGGGGAGGGGTGCATTTGATATCTGGGGCCAAGGGACAATGGTC7860.11453485152727706No Hit
GTGCAGCCACAGTTCGATTGATATCCACTTTGGTCCCAGGGCCGAAAGTG7770.11322338376169756No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7730.11264050919921778No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7680.11191191599611806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7650.11147476007425822No Hit
ACATAGTAGTATTGTGGGGAGGGGTGCATTTGATATCTGGGGCCAAGGGA7640.11132904143363828No Hit
GTTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGTTCC7640.11132904143363828No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATATCCACTTTGGTCC7610.11089188551177842No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7590.11060044823053855No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT7590.11060044823053855No Hit
GTCTACGGACATGCTGACGCGACTTTTGACGGACGGGTTGTAGTAGGTGC7580.1104547295899186No Hit
CTCCTCATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTT7570.11030901094929867No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7550.11001757366805877No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7480.10899754318371914No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC7470.1088518245430992No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7460.10870610590247926No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7410.10797751269937952No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7330.10681176357441996No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7290.10622888901194018No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7250.1056460144494604No Hit
GTCAGAGTGTTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGC7240.10550029580884046No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG7090.10331451619954128No Hit
ACCTACTACAACCCGTCCGTCAAAAGTCGCGTCAGCATGTCCGTAGACAC7080.10316879755892132No Hit
GAGTGTTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGG6970.10156589251210194No Hit
CCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGTTCCCAGACTCCTCATC6970.10156589251210194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)