FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283872
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTACACACTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGG38370.2988615687545176No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC38080.2966027766007826No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35410.27580631090949875No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA34670.270042496448244No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA34530.26895204506368237No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31210.24309276937264776No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC31130.24246965429575534No Hit
GACTTAGGACGGTGACCGTGGTCCCAGTTCCAAAGACCCCTGGGGTTCTT29990.2335902644500386No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC29870.23265559183470003No Hit
TCTTTGAAGAGCAGGCTCACCATCTTCAAGGACACCTCCAAAAGCCAGGT29610.23063046783479974No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA29400.2289947907579572No Hit
TCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGACTGTGTTCCCAGAGTT28640.22307519752747937No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA28600.22276363998903317No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGAAGAACCC28490.2219068567583061No Hit
ATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTACT28340.22073851598913286No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28100.2188691707584557No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT28060.2185576132200095No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA27590.2148968121432666No Hit
GACCTGCACCGTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGA26520.2065626479898308No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG26470.20617320106677303No Hit
ACATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTA25910.2018113955285262No Hit
GTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCTGAGCG25560.19908526706712196No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25430.19807270506717178No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25400.19783903691333715No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25260.19674858552877544No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25110.19558024475960217No Hit
GACTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAAC24820.19332145260586725No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24710.1924646693751402No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24400.19005009845218215No Hit
CCCCTGTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCT24150.1881028638368934No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23600.18381894768325815No Hit
CAGTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGAC22530.17548478352982227No Hit
ATATAGTGGGAGCTCCTCTTTTGGCTACTGGGGCCAGGGAACCCTGGTCA22170.17268076568380647No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21700.16901996460706364No Hit
GTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGAGCTGGATCCG21440.16699484060716333No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA21070.16411293337653599No Hit
CTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAACTG20650.16084157922285086No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20160.1570249993768849No Hit
TAAATATGCTTCCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGTTGG20020.15593454799232323No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19710.15351997706936518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG19540.15219585753096881No Hit
CTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGCA19380.150949627377184No Hit
GGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT18880.14705515814660652No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGA18870.14697726876199496No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG18850.14682148999277186No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18830.14666571122354877No Hit
CATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTAC18690.14557525983898706No Hit
GTATCAACGCAGAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGC18330.14277124199297125No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18280.1423817950699135No Hit
ACATGGACCCTGTGGACACAGCCACATATTACTGTGCACGGGAGCGATAT17770.13840943645472445No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17170.13373607337803145No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16850.13124361307046184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16440.1280501483013883No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16050.12501246230153784No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15780.12290944891702599No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15590.12142955060940655No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT15590.12142955060940655No Hit
GGATGAGGCTGACTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGG15580.121351661224795No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15420.1201054310710102No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC15410.12002754168639865No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCTCCTGCTCTGG15320.1193265372248947No Hit
CCCCAGGACAGACAGCCAGCATCTCCTGCTCTGGAGATGATTTGGGGGAT15310.11924864784028315No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15290.11909286907106004No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC15280.11901497968644849No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG15050.11722352384038283No Hit
GCCCTGGAGTGGCTTGCACACATTTTTTCGAATGACGAAAAATCCTACAG14960.11652251937887888No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14910.11613307245582113No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14790.11519839984048255No Hit
GAAGAACCCCAGGGGTCTTTGGAACTGGGACCACGGTCACCGTCCTAAGT14740.1148089529174248No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14710.11457528476359015No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA14620.1138742803020862No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT14570.11348483337902844No Hit
ATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCACCT14500.11293960768674759No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTC14370.11192704568679743No Hit
GTCTGGTCCTGTGCTGATGAAACCCACAGAGACCCTCACTCTGACCTGCA14050.10943458537922784No Hit
GGACAGAAGAACCCCAGGGGTCTTTGGAACTGGGACCACGGTCACCGTCC13830.10772101891777373No Hit
CAGTCACTCTGACCATCAGCGGGACCCAGCCTATGGATGAGGCTGACTAT13770.10725368261010444No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13760.10717579322549288No Hit
CTTCAAGGACACCTCCAAAAGCCAGGTGGTCCTTACAATGACCAACATGG13640.10624112061015428No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13610.10600745245631964No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13370.10413810722564243No Hit
ACACAGGGGACTGGCCTGGCTTCTGCTGATACCAGGAAGCATATTTATCC13340.10390443907180777No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13310.10367077091797312No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13270.10335921337952694No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13080.10187931507190748No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12940.10078886368734578No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)