FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452918
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG122460.49924212713184873No Hit
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA106820.4354813328452072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT104760.4270831719609053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC103310.4211718451248676No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA101380.41330366526724494No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA80000.3261421702641507No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC70990.28941040833815074No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT70130.2859043800078111No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC64440.2627075181477734No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT62740.25577699702966017No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG61720.25161868435879226No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61160.24933568916694324No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA57760.23547464693071682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55110.2246711875407168No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT54310.2214097658380753No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT53900.21973828721547156No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51510.20999478987883002No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT49740.20277889436173568No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA48570.1980090651216225No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG47730.1945845723338489No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46700.19038549189169798No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT45200.18427032619924513No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43380.1768505918257357No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT42140.1717953881866414No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41340.16853396648399988No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40320.16437565381313196No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC39720.16192958753615083No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT39560.16127730319562253No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC39370.16050271554124515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39340.1603804122273961No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39200.15980966342943384No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT38680.15768973932271685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37950.1547136920190565No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT37320.1521453224282263No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37180.15157457363026403No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTAGCCTGGTACC36800.15002539832150932No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA35940.1465193699911697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35730.14566324679422632No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC35710.14558171125166028No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT35430.14444021365573576No Hit
GTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT35160.14333948383109424No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC35030.14280950280441498No Hit
GCGTAGTAGTAGTAAAGGGGATAATAACCACTAGTATCACCTCTGGCACA34800.14187184406490555No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA34520.14073034646898103No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT34100.13901810007509424No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33810.13783583470788668No Hit
GTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCA33530.13669433711196216No Hit
GTACAAAATGTACCCAATCCACTCCAGGCCCCTCACTGGGTGCTGGCGGA33390.1361235883139999No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG32670.13318830878162255No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31100.12678776869018857No Hit
ATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGA30150.1229148304183018No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG30140.12287406264701876No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG29840.1216510295085282No Hit
GTGTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGG29490.12022415751362255No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29210.11908265991769802No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG28430.11590277375762255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC28350.1155766315873584No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG28300.11537279273094331No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTA28080.1144759017627169No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT27650.11272288759754709No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC27240.11105140897494331No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27120.11056219571954709No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC27090.11043989240569804No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCA26470.10791229058615087No Hit
TCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGC26230.1069338640753584No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATTTTGCAGTGTA25950.10579236647943388No Hit
GCCGTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTA25800.10518084991018858No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC25790.10514008213890558No Hit
ACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGC25040.10208249929267917No Hit
CATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAG24760.10094100169675464No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA24530.10000334295724521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)