FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390402
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA56300.4049188651915058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53780.38679461047955915No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT53500.384780804400454No Hit
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG52540.3778763264149505No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA52330.3763659718556216No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47280.34004554078604604No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT38840.27934367183016134No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC37320.2684115816864475No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA36360.26150710370094404No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG29780.21418266084197232No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA29250.21037081362080898No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC28190.20274711917848218No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27890.20058946980801234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26170.18821894675065196No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25340.18224945015901875No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT24690.17757454318966745No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG24460.1759203453389739No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24110.17340308774009244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23920.17203657647212822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.1697350838102937No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22520.1619675460766023No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT22490.16175178113955532No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT22440.16139217291114366No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA21640.15563844125655746No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21200.152473888846535No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA21020.15117929922425313No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20570.14794282516854837No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT20480.14729553035740742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20390.1466482355462665No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20210.14535364592398456No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19960.14355560478192636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA19760.14211717186827982No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19600.14096642553736258No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC19600.14096642553736258No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT19000.1366511267964229No Hit
GTACAAAATGTACCCAATCCACTCCAGGCCCCTCACTGGGTGCTGGCGGA18020.12960280551955478No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17720.12744515614908494No Hit
ATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGA17330.12464021196747417No Hit
GTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT17150.12334562234519225No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT17070.12277024917973364No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT16870.12133181626608708No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16860.12125989462040475No Hit
GCGTAGTAGTAGTAAAGGGGATAATAACCACTAGTATCACCTCTGGCACA16610.11946185347834655No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTAGCCTGGTACC16440.11823918550174697No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC16430.11816726385606466No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15970.11485886815467757No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15430.11097509928783186No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15380.11061549105942023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15360.11047164776805557No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15330.1102558828310086No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC15180.10917705814577366No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15180.10917705814577366No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC14590.10493368105051633No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14560.10471791611346934No Hit
GTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCA14550.10464599446778702No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14520.10443022953074002No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14450.10392677801096374No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCT14250.10248834509731719No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14250.10248834509731719No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG14110.10148144205776458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)