FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745291
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80090.45889195555354384No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT79150.45350603423727043No Hit
GTATGGTAGTTACCCCGAGACTTTTGGCCAGGGGACCAAGCTGGAGATCA73620.421820773727705No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT50380.2886624637381388No Hit
GAGTATTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCC48540.27811980924671015No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43160.24729400426633721No Hit
CTATAAGACATCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCA40540.23228218102310733No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA39800.22804220041242407No Hit
CTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT39800.22804220041242407No Hit
CTCCTGTACAGCTTCTGGATTCTTCTTTCGCGATTATGCTATGAACTGGG38840.22254168502559174No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35280.20214394046608847No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35030.2007115145841009No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT34200.1959558606559021No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT33740.19332019703304493No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC33610.19257533557441137No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32960.18885102828124364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT32400.18564239430559146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG32120.18403807731776534No Hit
CCTTCCACCCTGTCTGCATCTTTAGGAGACAGAGTCACCATCGCTTGCCG31960.1831213247532933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30940.1772770271547839No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29790.17068786809764103No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT28410.16278087722906953No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27920.15997332250037385No Hit
CAGTATGGTAGTTACCCCGAGACTTTTGGCCAGGGGACCAAGCTGGAGAT27300.15642090631304464No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27260.15619171817192662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA26070.1493733709736657No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25380.14541987553937996No Hit
TTTAAAAGGTGTCCAATGTGACGTGCAGGTGGTGGAGTCTGGGGGAGGCT24860.1424404297048458No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24130.13825774612944203No Hit
TTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGAGTATTAGTGG23400.13407506255403825No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA23220.1330437159190072No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC23030.13195507224869663No Hit
GTCTGCATCTTTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGA23000.1317831811428581No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA22980.13166858707229911No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC22360.1281161708849699No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC22250.12748590349689537No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG22160.12697023017937983No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG21830.1250794280151562No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG21790.12485023987403819No Hit
ACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC21720.12444916062708167No Hit
GTCTTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCA21700.12433456655652267No Hit
GTATTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT21510.12324592288621211No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA21430.12278754660397606No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20380.1167713578996282No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG20080.1150524468412431No Hit
GGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATAAGACA19470.11155732768919338No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG19440.11138543658335487No Hit
GATTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA19290.11052598105416231No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19180.10989571366608777No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA18980.10874977296049772No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC18930.10846328778410018No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCTTCTTTCGCGATTATGCT18720.10726005004323062No Hit
GTACAGTGGAAGGTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAG18620.10668707969043557No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC18530.10617140637292004No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG18260.10462438642037344No Hit
ACTTATTACTGCCAACAGTATGGTAGTTACCCCGAGACTTTTGGCCAGGG18240.10450979234981446No Hit
CTAATACTCTGACTGGCCCGGCAAGCGATGGTGACTCTGTCTCCTAAAGA18210.10433790124397593No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC18150.10399411903229891No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA17870.1023898020444728No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17850.1022752079739138No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT17560.1006135939508082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)