FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816656
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA134140.4762384898972399No Hit
GTCTATTACTGTGCGAGAGCTACCGGCTATGATAATAGTGGTTATTACTC125320.4449247618452519No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122330.434309337029442No Hit
GATTATTACTGTGCAGGATGGGATGACAGCCTGAATGGGGTATTCGGCGG113670.40356365846592557No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC76600.27195369260569985No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT73300.26023767190597646No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG72870.25871103890570946No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC70770.25125538936952185No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC70630.25075834606710934No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT69090.2452908697405718No Hit
GCTATACACTGGGTCCGCCAGGCTCCGGACAGGGGGCTGGAGTGGGTGAC66620.236521605762294No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC59280.2104623354786669No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58770.2086516777341642No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT57890.20552740554757132No Hit
ATGCTATACACTGGGTCCGCCAGGCTCCGGACAGGGGGCTGGAGTGGGTG57530.20424929419851057No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC57490.20410728182639273No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA56660.2011605251049471No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA56310.19991791684891586No Hit
CTATTATCATAGCCGGTAGCTCTCGCACAGTAATAGACAGCCGTGTCCTC55740.1978942405462364No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGGTGGTGGAGTCTGGGGGAGGCG55600.1973971972438239No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCATTCAGGCTG54660.1940599064990542No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG53210.1889119580097818No Hit
CAGTTATATCATATGATGGAGGCAATAAAAACTACGCAGACTCCGTGAAG52790.1874208281025443No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52740.18724331263739696No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51900.18426105282292193No Hit
GGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGG50550.1794681352639442No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT48010.17045034963446015No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA47650.16917223828539943No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCC47120.1672905743548378No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG47090.1671840650757494No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC46380.1646633454706574No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA45740.16239114751677167No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45370.16107753307468148No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA44960.15962190626047343No Hit
CATCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG44440.15777574542294126No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA43380.154012417561818No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41840.14854494123528042No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC41700.1480478979328679No Hit
GAAGTAATACTGTCAACTGGTACCAACAGCTCCCAGGAACGGCCCCCAAA40920.14527865667656967No Hit
ATATAACTGTCACCCACTCCAGCCCCCTGTCCGGAGCCTGGCGGACCCAG40550.14396504223447948No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT39720.1410182855130339No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA38840.137894013326441No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC38490.13665140507040974No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA38160.13547980300043738No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC38080.1351957782562017No Hit
GTGCAGGATGGGATGACAGCCTGAATGGGGTATTCGGCGGAGGGACCAAG37850.13437920711652399No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37680.1337756545350231No Hit
GTATTACTTCCGATGTTGGAGATGCTTCCAGAACAAGAGATGGTGACCCT37420.132852574116257No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37400.13278156793019807No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT37370.13267505865110968No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGAC37320.13249754318596235No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG37050.13153895967416682No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36860.13086440090660698No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36690.1302608483251061No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT36650.13011883595298823No Hit
TGATAATAGTGGTTATTACTCCGCCCTTGACCACTGGGGCCAGGGAACCC35810.12713657613851317No Hit
GGTTATTACTCCGCCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGT34980.12418981941706762No Hit
TTATTACTGTGCAGGATGGGATGACAGCCTGAATGGGGTATTCGGCGGAG34880.12383478848677297No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG34490.12245016785862384No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGG34420.12220164620741758No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT33820.12007146062564972No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT33500.11893536164870684No Hit
GTAATAACCACTATTATCATAGCCGGTAGCTCTCGCACAGTAATAGACAG33240.11801228122994074No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT32790.11641464204361483No Hit
GGATGGGATGACAGCCTGAATGGGGTATTCGGCGGAGGGACCAAGCTGAC31880.11318386057793356No Hit
GGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGGAT31750.1127223203685505No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT31490.11179923994978443No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG30920.10977556364710495No Hit
GTGTATAGCATAATTACTGAAGGTGAATCCAGAGGCCGCACAGGAGAGTC30570.10853295539107366No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT30490.10824893064683797No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA30370.10782289353048438No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC30270.10746786260018974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA29980.10643827290233525No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG29180.10359802545997807No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACCAGTT28880.10253293266909413No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28340.10061576564550305No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC28290.10043825018035571No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGGATGGGATGACAGCCTGA28260.10033174090126733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)