FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575805
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTACTGTGCGCGAGAGAACGCCGGTGAAGAGTTTGACTACTGGGG92250.5854150735655744No Hit
GTCATATATTATGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGA87150.5530506629944695No Hit
GTAATAAATAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA84400.5355992651375011No Hit
ATATTATGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAATG60860.3862152994818521No Hit
TTATTACTGTGCGCGAGAGAACGCCGGTGAAGAGTTTGACTACTGGGGCC51750.3284035778538588No Hit
ATATATGACACTGCTACCGCTGCTAATAAATGAGACCCATTCCGGCCCCT51020.3237710249681909No Hit
CAGTAATAAATAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA45000.2855683285685729No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40400.25637689942600767No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36180.2295969361691326No Hit
ACATAAGAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA35550.2255989795691726No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA33960.21550889862641637No Hit
CTATAGCATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGGCCGGAATGGG30940.19634409079803658No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29820.18923661239810763No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28370.1800349662553425No Hit
GATCTATGCTGCATCCATTTTACCAAGTGGGGTCCCATCAAGGTTCAGCG27030.1715313760268561No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26570.1686122331125996No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25630.16264702802694497No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23880.15154159302705603No Hit
GTGCTGGGTTTTCCTTATTGTTTTTTTAGCAGGTGTCCAGTGTGAAGTGC23860.15141467376991444No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22900.1453225494271182No Hit
CTCCAGAGACAATGCCAAGAGCTCACTGTATCTGCAAATGAACAGCCTGA22430.14233994688429089No Hit
GTCTACAACATAAGAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTG22220.14100729468430423No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA21700.13770739399862292No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21050.13358251814152133No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20970.13307484111295498No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGACTATAGCATGAACTGGG20680.13123451188440194No Hit
GTTGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATACGAGA20670.13117105225583114No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20430.1296480211701321No Hit
CTCTTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT19700.12501546828446414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19570.1241904931130438No Hit
GCATAATATATGACACTGCTACCGCTGCTAATAAATGAGACCCATTCCGG19510.12380973534161904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18770.1191137228273803No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18730.11885988431309712No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT18680.11854258617024313No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG18500.11740031285596886No Hit
GTGTCATATATTATGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGA18350.11644841842740694No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18290.11606766065598217No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18270.1159407413988406No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17480.11092743074174788No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17400.11041975371318152No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17390.11035629408461071No Hit
AGCATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGGCCGGAATGGGTCTC16940.107500610798925No Hit
ACTTATTACTGTCTACAACATAAGAGTTTCCCGTACACTTTTGGCCAGGG16830.10680255488464627No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16650.10566028157037197No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16490.10464492751323927No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16300.10343919457039417No Hit
GTCTGGGACAGAGTTCACTCTCACAATCAACAGCCTGCAGCCTGAAGATT16170.10261421939897386No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTATTGTTTTTTTAGCAGGTGTCCA16150.10248730014183226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)