FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952930
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATTATCTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA80780.8477013002004344No Hit
TAATTATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCT62360.654402736822222No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT53440.5607967007020453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41860.43927675694961854No Hit
ATATTACTGTCAACAGTATAATTATCTCCCCCTCACTTTCGGCGGAGGGA36170.379566180097174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28610.30023191630025287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25380.2663364570325208No Hit
CTAGGAGACAGGGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA23820.2499658946617275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23070.24209543198346153No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG22910.24041639994543143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21780.22855823617684407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21240.22289150304849256No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG20960.2199531969819399No Hit
ACATATTACTGTCAACAGTATAATTATCTCCCCCTCACTTTCGGCGGAGG20680.21701489091538725No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20540.21554573788211098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT19530.2049468481420461No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTCACGGTGAAAGT19360.20316287660163915No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC19320.20274311859213162No Hit
ACTGTCAACAGTATAATTATCTCCCCCTCACTTTCGGCGGAGGGACCAAG18850.19781096198041828No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18620.19539735342575004No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17700.1857429192070771No Hit
CTCTAGGAGACAGGGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGC17420.18280461314052449No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16530.1734649974289822No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTCACGGTGAAAGTAA16490.17304523941947467No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16360.17168102588857523No Hit
GTAATATTCAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16350.17157608638619837No Hit
GTCTCCATCCTCCCTGTCTGCATCTCTAGGAGACAGGGTCACCATCACTT16300.17105138887431395No Hit
ATTATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC16000.16790320380300755No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15120.15866852759384215No Hit
GATTTTACTTTCACCGTGAGCAGCCTGCAGCCTGAAGATTTTGCAACATA15070.15814383008195776No Hit
CAGTATAATTATCTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT14720.15447094749876697No Hit
GTACAGTGGAAGGTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAG14710.15436600799639008No Hit
GATCTACGATGCATCCAATTTGGAAATAGGGGTCCCATCAAGGTTCAGTG14320.15027336740369177No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14310.15016842790131488No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14120.14817457735615416No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13950.14639060581574723No Hit
GTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTCACGGTG13950.14639060581574723No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA13890.14576096880148595No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13890.14576096880148595No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13880.14565602929910906No Hit
ATACTATGGAGACTCCGTGAAGGGCCGATGCACGATCTCCAGAGACAATT13800.14481651328009404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13330.13988435666838067No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13190.13841520363510434No Hit
CTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA13130.13778556662084307No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13040.13684111109945116No Hit
GAATATTACTGTGCGAGAGCTTCTACAGCAACTACGAGGGTTGACTACTA12590.13211883349249157No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12420.13033486195208463No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC12230.12834101140692392No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12170.12771137439266264No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12140.12739655588553198No Hit
GGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC12080.1267669188712707No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11980.12571752384750193No Hit
AATAAATACTATGGAGACTCCGTGAAGGGCCGATGCACGATCTCCAGAGA11970.12561258434512504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11780.12361873379996431No Hit
CCGTAGTAGTAGTCAACCCTCGTAGTTGCTGTAGAAGCTCTCGCACAGTA11690.12267427827857241No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11230.11784706116923593No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10890.11427911808842203No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10880.11417417858604516No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10870.11406923908366827No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCA10710.11239020704563818No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10700.11228526754326132No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10660.11186550953375379No Hit
GTCTGCATCTCTAGGAGACAGGGTCACCATCACTTGCCAGGCGAGTCAGG10630.11155069102662316No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10610.11134081202186939No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10560.11081611450998499No Hit
ATTATCTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA10340.10850744545769363No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA10170.10672347391728668No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10130.10630371590777916No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC10100.10598889740064853No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9990.10483456287450285No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA9880.10368022834835718No Hit
CTACTACGGTGTGGACGTCTGGGGCCCAGGGACCACGGTCATCGTCTCCT9850.10336540984122652No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC9830.10315553083647278No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATT9780.10263083332458836No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9750.10231601481745774No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC9720.10200119631032709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)