FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785998
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAAAA195131.092554414954552No Hit
ATATTATAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCA106840.5982089565609816No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT102080.5715571909934949No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91110.5101349497591823No Hit
CTATAATATTGCTGACAGTAGTAAAGTGCCACATCTTCAGCCTGCAGGCT78190.4377944432188614No Hit
TTATAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAAC76950.43085154630632283No Hit
TTACTACTGTCAGCAATATTATAGTACTCCTTGGACGTTCGGCCAAGGGA67440.37760400627548296No Hit
GTACTATAATATTGCTGACAGTAGTAAAGTGCCACATCTTCAGCCTGCAG56760.31780550706103816No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAATTGGTGGAGTCTGGGGGAGGCG46210.2587348922003272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45420.2543115949737906No Hit
GTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAAAAACCAGGACAG45320.25375168393245684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45240.25330375509938985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42760.23941796127431272No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT41600.2329229931948412No Hit
CAGTAGTAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT41060.22989947357163892No Hit
GTATAAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCGCGCCCA38420.21511782208042787No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38160.2136620533729601No Hit
TTGTATTACTGTGTGAGAGATCGGAGTCCTAGTGGTTACGATGCCGATGC34960.19574490005028No Hit
CAGTAATACAAACCCGTGTCCTCAGCTCTCAGACTATTCATTTGCACAGA34670.19412115803041213No Hit
GTAGTAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA33900.18980984301214224No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA33800.18924993197080847No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33390.1869542967013401No Hit
CTCCAAAGCTGCTCATTTACTGGGCATCTTCCCGGGAATCCGGGGTCCCT33240.18611443013933945No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT33040.18499460805667195No Hit
ATAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGA32720.18320289272440393No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG31520.17648396022839893No Hit
GTATTACTGTGTGAGAGATCGGAGTCCTAGTGGTTACGATGCCGATGCTT31120.1742443160630639No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC30860.17278854735559612No Hit
AACCAGGACAGCCTCCAAAGCTGCTCATTTACTGGGCATCTTCCCGGGAA30250.16937309000346024No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGC29770.16668551700505824No Hit
GTAATACAAACCCGTGTCCTCAGCTCTCAGACTATTCATTTGCACAGATA29160.16327005965292235No Hit
CTACTTAGCTTGGTATCAGCAAAAACCAGGACAGCCTCCAAAGCTGCTCA29110.16299010413225548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA28720.16080645107105382No Hit
ATACAAACCCGTGTCCTCAGCTCTCAGACTATTCATTTGCACAGATACCG28560.1599105934049198No Hit
CTTTACTACTGTCAGCAATATTATAGTACTCCTTGGACGTTCGGCCAAGG28490.1595186556759862No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC28320.15856680690571884No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG27850.15593522501145018No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG27010.15123197226424664No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26380.147704532703844No Hit
CTCCCTGGCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCA26130.14630475510050964No Hit
ACTTAGCTTGGTATCAGCAAAAACCAGGACAGCCTCCAAAGCTGCTCATT25780.1443450664558415No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG25580.143225244373174No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC25200.1410975824161057No Hit
ATTCAGTGGCAGTGAGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC25120.14064965358303874No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT25110.14059366247890537No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24940.13964181370863799No Hit
GTCCCTGACCGATTCAGTGGCAGTGAGTCTGGGACAGATTTCACTCTCAC24690.13824203610530358No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC24660.13807406279290346No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24620.13785009837636997No Hit
GTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTT23860.13359477446223345No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23510.1316350858175653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23140.12956341496463042No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGAGGGCCACCATCAACT23030.12894751281916328No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC22710.1271557974868953No Hit
GAGTACTATAATATTGCTGACAGTAGTAAAGTGCCACATCTTCAGCCTGC22690.12704381527862854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22580.1264279131331614No Hit
GAACTACTTAGCTTGGTATCAGCAAAAACCAGGACAGCCTCCAAAGCTGC22240.12452421559262664No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTA22040.12340439350995913No Hit
GGTTTGTATTACTGTGTGAGAGATCGGAGTCCTAGTGGTTACGATGCCGA21920.12273250026035865No Hit
GCTCATTTACTGGGCATCTTCCCGGGAATCCGGGGTCCCTGACCGATTCA21170.1185331674503555No Hit
CTCCTGTGCAGCCTCTGGATTCAGGTTCAGAAGTCATGCGATCCACTGGG20750.11618154107675373No Hit
CAATAAGAACTACTTAGCTTGGTATCAGCAAAAACCAGGACAGCCTCCAA20740.11612554997262035No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG20720.11601356776435359No Hit
GTCCCAGACTCACTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGA20580.11522969230648634No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20370.11405387911968548No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20340.11388590580728535No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20180.11299004814115134No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG19990.1119262171626172No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19940.11164626164195032No Hit
TCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATG19890.11136630612128345No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC19500.10918265306008182No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC19430.10879071533114819No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACTC19100.1069430088947468No Hit
CCCTGGCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCAGC19050.10666305337407993No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA19010.10643908895754642No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18860.10559922239554578No Hit
TCCAAGGAGTACTATAATATTGCTGACAGTAGTAAAGTGCCACATCTTCA18820.10537525797901229No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC18560.10391948927154454No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18470.10341556933434416No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT18280.10235173835581003No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA18240.10212777393927652No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18090.10128790737727589No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA17900.10022407639874177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)