FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005108303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396399
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAATGGTGCTCGAGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTA142430.5943501061384185No Hit
CCATTATAGGAGAGCAAGCAGTAATAGTTAGCCTCATCCTCAGGCTGCGC105330.4395344848666687No Hit
GTAATATACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG101290.4226758565664566No Hit
GTATATTACTGTGCGAGACAACATCAAGAGGCTTTAGGCTATATCAGTGG92270.3850360478367751No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC84700.35344698441286276No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGACAAAGCTG77720.324319948389229No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT69110.2883910400563512No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACTCGAGCACCATTA68900.287514725218964No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA65880.2749124832717757No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT62820.26214332421270414No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA62160.2593891918666299No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCCCCAAGGAG61940.25847114775127183No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA60390.2520031096657944No Hit
CTATTACTGCTTGCTCTCCTATAATGGTGCTCGAGTGTTCGGCGGAGGGA58090.2424053757325053No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT57900.24161251945105966No Hit
GATTTATGATACAAGTAAGAAACACTCCTGGACCCCTGCCCGGTTCTCAG56500.23577042053514458No Hit
AACTATTACTGCTTGCTCTCCTATAATGGTGCTCGAGTGTTCGGCGGAGG56320.23501929353166984No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACTCG55480.23151403418212074No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51390.21444675949205452No Hit
TCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTA50550.21094150014250548No Hit
GCCTGAGGATGAGGCTAACTATTACTGCTTGCTCTCCTATAATGGTGCTC50530.21085804158656385No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50320.20998172674917656No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50010.20868811913208107No Hit
CTCCTATAATGGTGCTCGAGTGTTCGGCGGAGGGACCAAGGTGACCGTCC49820.20789526285063548No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA49170.20518285978253203No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG48860.20388925216543655No Hit
TTATAGGAGAGCAAGCAGTAATAGTTAGCCTCATCCTCAGGCTGCGCACC48620.20288774949413685No Hit
CTCACTGACTGTGTCCCCACGAGGGACAGTCACTCTCACCTGTGGCTCCA47840.1996328658124127No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT47420.1978802361376382No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46840.19545993801533051No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA46380.1935403912286727No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA45000.18778175086869925No Hit
GTAATAGTTAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG44230.18456859646494594No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA43960.18344190595973375No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC43560.18177273484090087No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43490.1814806298951051No Hit
GTCCAGGAGTGTTTCTTACTTGTATCATAAATCAGTGTCCTGGGGGCTTG43020.17951935383047649No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC42550.17755807776584784No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42230.17622274087078152No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42210.17613928231483988No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG41170.17179943740587442No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41070.1713821446261662No Hit
ATATACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTG40800.170255454120954No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA40540.1691704928937126No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40010.16695884116125903No Hit
ATATTACTGTGCGAGACAACATCAAGAGGCTTTAGGCTATATCAGTGGCT39850.16629117271372587No Hit
GCCGTATATTACTGTGCGAGACAACATCAAGAGGCTTTAGGCTATATCAG39820.16616598487981343No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38640.1612419300792564No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG38450.1604490737978108No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38400.1602404274079567No Hit
GATATAGCCTAAAGCCTCTTGATGTTGTCTCGCACAGTAATATACGGCCG37950.1583626098992697No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG37930.15827915134332807No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA37750.15752802433985325No Hit
CTATAATGGTGCTCGAGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAG37170.15510772621754557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT37030.15452351632595407No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA36910.1540227649903042No Hit
GTAAGAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGG36030.15035058852887187No Hit
TAGTTAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTT35960.1500584835830761No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT35400.14772164401671006No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC33990.14183781582282415No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT33780.1409615009854369No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA33380.139292329866604No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33110.1381656393613918No Hit
CAGTAATAGTTAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGC32720.13653819752052976No Hit
CACTGACTGTGTCCCCACGAGGGACAGTCACTCTCACCTGTGGCTCCAGC32400.13520286062546344No Hit
GGCTAACTATTACTGCTTGCTCTCCTATAATGGTGCTCGAGTGTTCGGCG32090.13390925300836798No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTATATTA32050.13374233589648468No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCC31230.13032053510287728No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACTCGAGCACCATTAT31200.1301953472689648No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC30980.12927730315360672No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC30950.12915211531969426No Hit
GTTCTCAGGCTCCCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTG30840.12869309326201522No Hit
GGTTACACCTTTACCAACTACGGTATCATCTGGATGCGGCAGGCCCCTGG30680.12802542481448206No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30510.12731602708897807No Hit
GTTTCTTACTTGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTC30130.12573031452608685No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCACGAGGGACAGTCAC29950.12497918752261206No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC29300.12226678445450861No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG29100.12143219889509217No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA29070.12130701106117972No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28950.12080625972552983No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG28840.1203472376678508No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28590.11930400571858026No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG28540.11909535932872614No Hit
ACTATGCACAGAAGCTCCACGACAGAGTCACCATGACCACAGAAACATCC28310.11813558593539723No Hit
GGCTATATCAGTGGCTGGTTCCGAATCGACCCCTGGGGCCAGGGAACCCT28010.11688370759627258No Hit
TCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACAC27910.11646641481656435No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG27770.11588220492497285No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27420.11442168019599407No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT27350.11412957525019832No Hit
TTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCACGAG26740.11158408929397817No Hit
GGATGGATCAGCCCTTACAATGGTTACACAAACTATGCACAGAAGCTCCA26490.11054085734470763No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG26190.10928897900558297No Hit
CTCCACGACAGAGTCACCATGACCACAGAAACATCCACGACCACAGCCTA26170.10920552044964131No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG26070.10878822766993308No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC25970.10837093489022487No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25790.10761980788675009No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC25780.10757807860877927No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC25320.10565853182212144No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25240.10532469759835486No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG25060.10457357059488007No Hit
TCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTT25040.10449011203893842No Hit
CCCCTGGACAAGCGCTTGAGTGGATGGGATGGATCAGCCCTTACAATGGT25030.1044483827609676No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC25010.10436492420502597No Hit
ATGCACAGAAGCTCCACGACAGAGTCACCATGACCACAGAAACATCCACG24830.10361379720155116No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC24610.10269575308619308No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT24550.10244537741836814No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA24460.10206981391663074No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24140.10073447702156443No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA24080.1004841013537395No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCC24030.1002754549638854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)