FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080052
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACGGCCGTGTCTTCAATTCTCAGGGCGCCAATTTCCATGTAGG129050.6204171818781454No Hit
CAGTAACACCGACCGGCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCA100950.4853244053514047No Hit
ATATAAGAGTTACTGTAGTCTCTCGCACAGTAGTAGACGGCCGTGTCTTC74100.3562410939726507No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC66130.3179247441890876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65350.3141748379367439No Hit
TCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGGGGGACCAAGCT63710.30629041966258536No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT62440.3001848030722309No Hit
GTCTACTACTGTGCGAGAGACTACAGTAACTCTTATATGGATGTCTGGGG58730.28234871051300636No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57880.2782622742123755No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA57150.2747527465659512No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT55630.2674452369459994No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA54910.2639837850207591No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT54160.2603781059319671No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG53280.2561474424677845No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC53060.25508977660173876No Hit
CTCTTATATGGATGTCTGGGGCCAAGGGACCACGGTCACCGTCTCATCAG52100.250474507368085No Hit
CTCCTGCACTGGGAACAGCTCCAACATCGGGGCGGGTTATGATGTCCACT51330.24677267683692522No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50440.2424939376515587No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA47250.22715778259389668No Hit
ATTATTGCCAGTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGG45790.22013872730104822No Hit
GTCATAGGACTGGCAATAATAATCAGCCTCATCCTCACTCTGGAGCCCAG45060.21662919965462404No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA42840.20595638955179965No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40840.19634124531502095No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG40710.19571626093963035No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40420.19432206502529745No Hit
TCATTGGACTCGTCCGCGGTGATCGTGAGTCTGCCCTGAAATCTCTGTGC39930.19196635468728668No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC39030.18763953978073625No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC39010.18754338833836848No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA38450.18485114795207044No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38290.18408193641312814No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA35990.17302452054083262No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC35520.17076496164518964No Hit
GTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGGGGGACCAAGC35410.1702361287121668No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA34660.1666304496233748No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34550.16610161669035195No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGGCAGAGGGTCTC34520.16595738952680028No Hit
GTATGGAACAGCAAAATACGCACAGAGATTTCAGGGCAGACTCACGATCA34300.16489972366075464No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34030.1636016791887895No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCCCCGCCGAACCCCCA33310.16014022726354918No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG32980.1585537284644807No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT32670.15706338110778No Hit
CTCCAGACCTTGTCCAGGGGCCTGTCGCACCCAACTTATAGCATAACTCT32520.15634224529002158No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA32460.15605379096291824No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT32100.15432306500029808No Hit
GACCTAAGACGGTCAGCTTGGTCCCCCCGCCGAACCCCCAACCACTGAGG31870.15321732341306854No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA31570.1517750517775517No Hit
GCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCGCCTCAG31340.15066931019032218No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31120.1496116443242765No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT30940.14874628134296644No Hit
CCTCCAAGAGTTATGCTATAAGTTGGGTGCGACAGGCCCCTGGACAAGGT30850.14831359985231138No Hit
GTGTCATCCCTATGTATGGAACAGCAAAATACGCACAGAGATTTCAGGGC30520.14672710105324288No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT30510.146679025332059No Hit
GTCTGGGGCTGAAATGAAGAAGTCTGGGTCCTCGGTGAAGGTCTCCTGCA30130.14485214792707105No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29840.14345795201273814No Hit
ATGTATGGAACAGCAAAATACGCACAGAGATTTCAGGGCAGACTCACGAT29700.14278489191616364No Hit
GTGTTACTGTGAATGAGGAGTTTGGGGGCTGTTCCTGGCAGCTGCTGGTA29580.1422079832619569No Hit
ATCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCC29080.13980419720276221No Hit
CTCTTGGAGGTGCGTCCAGAGGCCTTGCAGGAGACCTTCACCGAGGACCC28990.1393715157121072No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT28780.13836192556724544No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28350.136294669556338No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28200.1355735337385796No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28170.1354293065750279No Hit
GATTATTATTGCCAGTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGG28070.134948549363189No Hit
GGTCTGGAGTGGATGGGCAGTGTCATCCCTATGTATGGAACAGCAAAATA27740.1333620505641205No Hit
GTTCCATACATAGGGATGACACTGCCCATCCACTCCAGACCTTGTCCAGG27680.13307359623701717No Hit
GCACAGAATCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT27590.1326409147463621No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27140.1304775072930869No Hit
TCCATACATAGGGATGACACTGCCCATCCACTCCAGACCTTGTCCAGGGG27070.13014097724479964No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTGCCAGGAACAGCCCCCAAACT26960.12961214431177684No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC26900.12932368998467345No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT26860.12913138709993788No Hit
CCTCTAAAGAAGGCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG26630.12802564551270834No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26310.12648722243482374No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC26250.12619876810772038No Hit
GGGCAGAGGGTCTCCATCTCCTGCACTGGGAACAGCTCCAACATCGGGGC26240.1261506923865365No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA25610.1231219219519512No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24600.11826627411237797No Hit
GGTACCAGCAGCTGCCAGGAACAGCCCCCAAACTCCTCATTCACAGTAAC24460.11759321401580344No Hit
GGGTTATGATGTCCACTGGTACCAGCAGCTGCCAGGAACAGCCCCCAAAC24360.11711245680396452No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC24330.11696822964041283No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG24210.11639132098620611No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23800.11442021641766649No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23750.11417983781174701No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23000.11057415872295501No Hit
CTGCACAGAATCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT22970.11042993155940334No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22590.10860305415441537No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA22350.10744923684600192No Hit
CATCCACACTCCTCCTCTAAAGAAGGCCCTGGGAGCACAGCTCATCACCA22230.10687232819179521No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG22020.10586273804693344No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21910.10533390511391061No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC21730.10446854213260054No Hit
ATAATAATCAGCCTCATCCTCACTCTGGAGCCCAGTGATGGCCAGGGAAG21630.10398778492076159No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC21500.10336280054537098No Hit
GTCCAATGATATCGCCTACATGGAAATTGGCGCCCTGAGAATTGAAGACA21450.10312242193945152No Hit
CTCTGGAGCCCAGTGATGGCCAGGGAAGCTGAGGCGCCAGACTTGGAGCC21420.10297819477589983No Hit
GCCAGGAACAGCCCCCAAACTCCTCATTCACAGTAACACCGACCGGCCCT21070.10129554453446356No Hit
GTCCAGGGGCCTGTCGCACCCAACTTATAGCATAACTCTTGGAGGTGCGT21050.10119939309209579No Hit
CAGGAATACCCTGCCTCATGCGTATGCAAATACCCTGAGGTCTTCTGAGA20950.10071863588025684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)