FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853668
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAGTCGAGT70990.24876755109564253No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC59780.2094847753838218No Hit
GATTATTACTGTAGCTCATATGCAACTACCACCGCTGTGCTATTCGGCGG59090.20706683468434312No Hit
GTAATAAATGGCCGTGTCCGCAGCGTTCACAGAGCTCAGCCTCAGGGAGA52330.1833780243532184No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAAG51910.18190623436223136No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA49220.1724797698961477No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA46650.16347381685606033No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGTAGCTCATAT46640.16343877423722733No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACAGCGGTGGTA41930.14693370076687268No Hit
ATTTATTACTGTGCGAGAGACTTTGTGAAGTCTAGGAGTCCCTGGTCGTG41840.14661831719737545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT41750.14630293362787822No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT41650.14595250743954796No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39550.13859355748461277No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGGC38540.1350542529824773No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC37220.13042862729651802No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37190.13032349944001895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36660.12846624064186865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35160.12320984781691494No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC35050.12282437900975167No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34340.1203363530726069No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34090.11946028760178129No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTTTAACTATGTCTCCT33610.11777824189779611No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33500.11739277309063283No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA33320.11676200595163838No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA33060.11585089786197973No Hit
GGGTATATCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAG32200.11283723264233962No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT32060.11234663597867726No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG31040.10877228885770875No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30890.10824664957521338No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30680.10751075457971986No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT30590.10719537101022264No Hit
GTCTAGGAGTCCCTGGTCGTGGGGTTACAACTTGAGGCAAAGATATCACT28770.10081761438261214No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28630.10032701771894978No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA28580.10015180462478465No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28580.10015180462478465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)