FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853668
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111470.3906200721317266No Hit
ATCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAGTCGAGT75710.2653076671848302No Hit
GATTATTACTGTAGCTCATATGCAACTACCACCGCTGTGCTATTCGGCGG62500.2190163677064045No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC58020.20331727446920947No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAAG51610.18085495579724062No Hit
GTAATAAATGGCCGTGTCCGCAGCGTTCACAGAGCTCAGCCTCAGGGAGA49060.1719190879948193No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGTAGCTCATAT47460.16631226898153534No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43740.15327641477565015No Hit
ATTTATTACTGTGCGAGAGACTTTGTGAAGTCTAGGAGTCCCTGGTCGTG43150.15120890026450168No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC41760.14633797624671124No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT41290.1446909731615591No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41240.14451576006739397No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40900.14332431102707113No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT40420.14164226532308594No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA39670.13901406891060908No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGGC39580.13869868534111188No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC36460.12776538826520814No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACAGCGGTGGTA36300.12720470636387976No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG35200.12335001829224704No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT34390.12051156616677203No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTTTAACTATGTCTCCT32670.11448423572749178No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32550.11406372430149549No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA31640.11087484598769023No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31490.11034920670519487No Hit
GGGTATATCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAG31450.11020903622986275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30720.10765092505505196No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC30460.10673981696539331No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30280.10610904982639886No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30080.10540819744973837No Hit
GTCATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGCCTGCCGGA29630.10383127960225226No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG29070.10186889294760287No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC29040.1017637650911038No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28980.10155350937810566No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT28910.10130821104627448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)