FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005108391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514574
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAACACCGACCGGCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCA111410.44305715401495444No Hit
GTAGTAGACGGCCGTGTCTTCAATTCTCAGGGCGCCAATTTCCATGTAGG108540.43164368994509605No Hit
GTCATAGGACTGGCAATAATAATCAGCCTCATCCTCACTCTGGAGCCCAG92740.36880998530963893No Hit
TCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGGGGGACCAAGCT84020.3341321432576651No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA82260.3271329457792851No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG81780.3252240737397269No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA81330.3234345062026411No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT80010.31818510809385603No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC78350.31158359229038396No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT72490.2882794461407777No Hit
ATATAAGAGTTACTGTAGTCTCTCGCACAGTAGTAGACGGCCGTGTCTTC69660.27702505474088257No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT68300.27161658396213434No Hit
ATTATTGCCAGTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGG63440.25228925456160767No Hit
GTGTTACTGTGAATGAGGAGTTTGGGGGCTGTTCCTGGCAGCTGCTGGTA56650.2252866688353574No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG56340.22405385564314273No Hit
CTCTTATATGGATGTCTGGGGCCAAGGGACCACGGTCACCGTCTCATCAG54520.21681604915981795No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT52820.21005546068638267No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG52800.20997592435140106No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52760.20981685168143788No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA52530.2089021838291496No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50970.20269834970058548No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50330.20015318698117457No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA50100.19923851912888624No Hit
GACCTAAGACGGTCAGCTTGGTCCCCCCGCCGAACCCCCAACCACTGAGG49630.19736941525681884No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49630.19736941525681884No Hit
ATAATAATCAGCCTCATCCTCACTCTGGAGCCCAGTGATGGCCAGGGAAG49600.19725011075434648No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49490.1968126609119477No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG49170.19554007955224226No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA49100.1952617023798067No Hit
GTCTACTACTGTGCGAGAGACTACAGTAACTCTTATATGGATGTCTGGGG48960.19470494803493554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA48750.19386981651762883No Hit
GTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGGCGGGGGGACCAAGC47650.18949531809364129No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA46980.186830850871758No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCCCCGCCGAACCCCCA46550.18512081966965377No Hit
CCTCTAAAGAAGGCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG44990.17891698554108965No Hit
CTCTGGAGCCCAGTGATGGCCAGGGAAGCTGAGGCGCCAGACTTGGAGCC44920.1786386083686541No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT42710.16984984335318826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41820.16631047644650745No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC41200.1638448500620781No Hit
CTCCTGCACTGGGAACAGCTCCAACATCGGGGCGGGTTATGATGTCCACT41190.1638050818945873No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40600.16145876001263038No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC40540.16122015100768558No Hit
GGCAATAATAATCAGCCTCATCCTCACTCTGGAGCCCAGTGATGGCCAGG40540.16122015100768558No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA39660.15772055226849557No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT38780.15422095352930557No Hit
GATTATTATTGCCAGTCCTATGACAGCAGCCTCAGTGGTTGGGGGTTCGG38760.15414141719432398No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA37220.14801711940074144No Hit
GTATGGAACAGCAAAATACGCACAGAGATTTCAGGGCAGACTCACGATCA36740.14610824736118325No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36540.14531288401136735No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT36480.14507427500642256No Hit
CCTCCAAGAGTTATGCTATAAGTTGGGTGCGACAGGCCCCTGGACAAGGT36420.1448356660014778No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC35500.14117699459232458No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG35350.14058047207996263No Hit
TCATAACCCGCCCCGATGTTGGAGCTGTTCCCAGTGCAGGAGATGGAGAC34770.1382739183654965No Hit
CATCCACACTCCTCCTCTAAAGAAGGCCCTGGGAGCACAGCTCATCACCA34730.1381148456955333No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34580.1375183231831714No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC34420.13688203250331865No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA33920.1348936241287789No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT33820.1344959424538709No Hit
GTCTGGGGCTGAAATGAAGAAGTCTGGGTCCTCGGTGAAGGTCTCCTGCA33380.1327461430842759No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32910.1308770392122085No Hit
TCCTCACTCTGGAGCCCAGTGATGGCCAGGGAAGCTGAGGCGCCAGACTT32820.13051912570479135No Hit
GCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCGCCTCAG32070.12753651314298167No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31900.12686045429563814No Hit
TCATTGGACTCGTCCGCGGTGATCGTGAGTCTGCCCTGAAATCTCTGTGC31850.12666161345818416No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT31510.12530949576349712No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG31460.12511065492604312No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA31110.12371876906386528No Hit
GTGTCATCCCTATGTATGGAACAGCAAAATACGCACAGAGATTTCAGGGC30700.12208827419674267No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30590.12165082435434392No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC30580.12161105618685311No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA30020.11938403880736856No Hit
ATGTATGGAACAGCAAAATACGCACAGAGATTTCAGGGCAGACTCACGAT29980.11922496613740538No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29510.11735586226533799No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG29160.11596397640316013No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT29060.11556629472825218No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC27720.11023736028448557No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG27480.10928292426470645No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG27350.10876593808732613No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCA27210.10820918374245499No Hit
GGGCAGAGGGTCTCCATCTCCTGCACTGGGAACAGCTCCAACATCGGGGC26670.10606170269795202No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26610.10582309369300724No Hit
GCCAGGAACAGCCCCCAAACTCCTCATTCACAGTAACACCGACCGGCCCT26560.10562425285555327No Hit
CCTCATTCACAGTAACACCGACCGGCCCTCAGGGGTCCCTGCCCGATTCT26470.10526633934813612No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG25890.10295978563366996No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG25870.10288024929868836No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTGCCAGGAACAGCCCCCAAACT25560.1016474361064737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)